滋养层细胞Fc受体介导丙型肝炎病毒的跨膜转运研究

After detecting some placentae of hepatitis C patients in various pregnant period with the methods of immunohistochemistry 、hybridization in situ、binary and multiplex immunostaining, we found the expressing of the HCVcorrelation antigen-NS3、NS5 and HCV RNA in the trophoblastic cell of term placentae，and that the HCV immune complex was able to combine the Fc receptor-CD16 on the surface of trophoblastic cell. We isolated and identified the trophoblastic cell. The trophoblastic cell can be infected by HCV in vitro, which indicated that HCV NS3、NS5 and the reverse strand of HCV RNA did not express or the expressing of HCV RNA delayed obviously with the absence of anti-HCV and the blocking of CD16. These results demonstrated that the transmission of HCV in uterus occurred probablely in the ultimate pregnant period, and firstly CD16 mediated the HCV immune complex to enter the trophoblastic cell through transmembrane transporting, and the anti-HCV enhanced the infection of HCV. If the way was blocked the access to trophoblastic cell、replication and release of HCV would be affected seriously. This study established reliably the evidence for the controlling of the mother-to-infant transmission of HCV farther, and offered a new method for the protection and treatment of the hapetitis C through effective vaccine.

在我科既往HCV系列研究的基础上，进一步应用单克隆抗体免疫组化、原位杂交及免疫三重昙堑燃际跫觳馊颂ヅ套萄阆赴砻鍵gG Fc受体和HCV免复合物，并首次应用"抗体依赖的HCV感染增强作用实验"证实Fc受体介导的HCV免疫复合物的跨膜转运机制，了解HCV在胎盘组械拇嬖谧刺突虮泶锓绞剑徊讲扇∠嘤Υ胧┛刂艸CV宫内传播提供理论依据。