白术七物颗粒对气阴两虚型STC大鼠多机理干预机制研究

The slow transmission modes constipation (slow transit constipation STC) is a kind of gastro-intestinal tract transmission function barrier stubborn constipation, approximately composes the constipation 16% ~40%, the research indicated that the STC cause of disease and the pathogenesis and the intestines nervous system, the intestines nerve hands over the nature, the Cajal cell, the gastrointestinal hormone as well as the spirit, the psychological factor and so on concerns. The symptom is stubborn, treatment difficulty. The Bai Zhu seven pellets are we act according to many year clinical researches, take the Chinese medicine theory as the instruction group side development's one kind of traditional Chinese medicine compound prescription preparation, the earlier period research discovered that it has the good treatment result, and has many spot intervention mechanism, simultaneously non-toxic side effect. This project plans on the STC big mouse model, through determines the Bai Zhu seven pellets after the STC big mouse treatment intestinal tract advance rate; Observes the Bai Zhu seven pellets to the STC big mouse colon intermuscular nerve plexus in VIP, the SP influence; Observes the Bai Zhu seven pellets to the STC big mouse intestinal tract intermuscular nerve plexus in the NOS expression influence; Observes the Bai Zhu seven pellets to organize the Cajal intersititial cell and the C-KIT expression influence to the STC big mouse colon, proves the Bai Zhu seven pellet multi-pharmacology mechanism coordination treatment colon slow transmission modes constipation the hypothesis.

慢传输型便秘(slow transit constipation STC) 是一类胃肠道传输功能障碍的顽固性便秘,约占便秘的 16% ～40%，研究表明 ,STC的病因及发病机理与肠神经系统、肠神经递质、Cajal 细胞、胃肠激素以及精神、心理因素等有关。症状顽固 ,治疗困难。白术七物颗粒是我们根据多年的临床研究，以中医理论为指导组方研制的一种中药复方制剂，前期研究发现其具有良好的治疗效果，且具有多位点干预机制，同时无毒副作用。本项目拟在STC大鼠模型上，通过测定白术七物颗粒对STC大鼠治疗后肠道推进率；观察白术七物颗粒对STC大鼠结肠肌间神经丛内VIP、SP的影响；对STC大鼠肠道肌间神经丛内NOS及结肠组织Cajal间质细胞及C-KIT表达的影响，以论证白术七物颗粒多药理机理协同治疗气阴两虚型结肠慢传输型便秘的假说。

中医药是我国传统文化中的瑰宝，中医药治疗已成为现代综合治疗的重要手段之一。中药复方是在中医辨证论治的基础上进行的组方，也是最能体现出中医的特色与优势，同时中药复方也是很复杂的，研究表明，中药复方可通过多种方式产生治疗作用，而目前中药复方治疗疾病机制阐述尚未完全阐明，因此有必要对中药复方机制上做进一步的研究和探讨。在进行中药复方作用机制研究时，要选择疗效明确、有效成分的结构和活性基本清楚的中药复方。本课题组的前期研究已经论证了白术七物颗粒是一种有效治疗结肠慢传输型便秘的中药复方，本次研究是在前期基础上深入研究其作用机制。在研究过程中本课题组根据新的发现以及国内外最新研究进展，探讨中药复方制剂白术七物颗粒治疗结肠慢传输型便秘的多药理协同机理。本项目实验证实白术七物颗粒通过对气阴两虚型STC大鼠肠道推进率的推进作用和对肠道肌间丛神经元特异性烯醇化酶的影响、对结肠肌间神经丛内VIP的抑制及对SP的升高、对肠道肌间神经丛内NOS的抑制、对结肠组织Cajal间质细胞的影响来治疗结肠慢传输型便秘的干预机制。随着新型药物及非药物治疗方法的应用，结肠慢传输型便秘的治疗得到迅速发展。如现代的生物反馈治疗、心理治疗及手术治疗。但各种治疗都有自身的缺陷。西药毒副作用大，长期使用则产生依赖性，且使病情逐渐加重，愈加顽固。手术治疗患者损伤大，术后并发症多，巨大的创伤及术后并发症带来的痛苦常常让患者望而却步。所以目前中药复方治疗STC的应用前景广阔。