氧化石墨烯和石墨烯量子点与细胞在分子水平的相互作用及应用研究

Graphene oxide (GO) and graphene quantum dots (GQDs) have shown potential applications in drug delivery, gene transfection, and cell imaging due to their unique chemical structures, size, and chemical and physical properties. However, whether they can find real applications in these fields is determined by how they interact with cells, one of the key questions that has been barely explored. The question is also essential to discover their new bio-medical applications. There are numerous research concerning of this issue in literature, however, most of them focused on the cytotoxicity of the graphene materials. The knowledge of their interaction with cells at molecular level is lacking. Herein, high quality graphene materials will be prepared, and their interaction with cell membrane, membrane protein, and nucleus will be explored at the molecular level. Further, combining with our previous research results of graphene materials with DNA at molecular level, drug delivery and drug activity enhancement by graphene materials will be explored for development of anti-cancer pharmaceutics. The project will not only allow us to understand the interaction between graphene materials and cells, which will accelerate their applications in bio-medical field, but also to realize the biological effect of the nano materials at molecular level, thus will be important to basic scientific research and practical bio-medical application.

氧化石墨烯（GO）和石墨烯量子点（GQDs），因其独特的结构和理化性质，已在药物输送，基因导入、细胞成像等生物医药领域显示出潜在的应用价值。然而，GO和GQDs在生物医药领域能否具有实际应用，关键的科学问题之一是它们如何与细胞作用。这一基础科学问题的探究对拓展它们在生物医药领域的应用，预测它们的副作用等都非常必要。但是，目前鲜有相关的研究报道。本项目拟在课题组前期工作的基础上，制备侧向尺寸和荷电特性可控的GO和GQDs，系统考察它们与细胞膜，膜蛋白以及细胞核的作用。在此基础上，结合课题组前期有关GO和GQDs与DNA的研究结果，进一步探索它们在抗癌药物的定点输送、增强抗癌药物活性等领域的应用。项目的实施不仅有助于了解石墨烯类材料与细胞的相互作用机理，为它们在生物医药领域的应用奠定基础，也有助于在细胞水平上深入理解纳米材料的生物学效应，具有重要的科学意义和广阔的应用前景。

氧化石墨烯（GO）和石墨烯量子点（GQDs），因其独特的结构和理化性质，已在药物输送，基因导入、细胞成像等生物医药领域显示出潜在的应用价值。然而，GO和GQDs在生物医药领域能否具有实际应用，关键的科学问题之一是它们如何与细胞作用。这一基础科学问题的探究对拓展它们在生物医药领域的应用，预测它们的副作用等都非常必要。但是，目前鲜有相关的研究报道。本项目拟在课题组前期工作的基础上，制备侧向尺寸和荷电特性可控的GO和GQDs，系统考察它们与细胞膜，膜蛋白以及细胞核的作用。在此基础上，结合课题组前期有关GO和GQDs与DNA的研究结果，进一步探索它们在抗癌药物的定点输送、增强抗癌药物活性等领域的应用。项目的实施不仅有助于了解石墨烯类材料与细胞的相互作用机理，为它们在生物医药领域的应用奠定基础，也有助于在细胞水平上深入理解纳米材料的生物学效应，具有重要的科学意义和广阔的应用前景。