大气压等离子体射流内不同活性粒子在灭菌过程中贡献权重的研究

Plasma sterilization is one of the most important directions in the health care application of atmospheric pressure plasma jet (APPJ). In recent years, the study results showed that the active species (especially for the oxygen active species) is the primary cause of the sterilization in APPJ. However, for lack of the advanced plasma diagnostic technique in the past, there is still not a clear recognition for the proportion of sterilization for different active species in APPJ. In this project, a portable APPJ driven by lithium battery will be as the plasma source. Various advanced diagnostic techniques, such as laser-induced fluorescence spectroscopy, cavity ring-down spectroscopy, absorption spectroscopy, etc., will be applied to determine the absolute concentration of several active species of the portable APPJ, such as OH radical, O atom, O3, NO, NO2, etc., and the space distribution of these species in plasma jet. Meanwhile, the control methods and production mechanisms of these active species concentration will be also studied. Then based on these work, three different type germs will be inactivated by APPJ at different experiment conditions. Finally the proportion of sterilization for different active species in APPJ and the optimal working parameters of APPJ can be get at the same time. This project can provide the scale of sterilization using plasma jet and improve the practical application of plasma sterilization.

等离子灭菌消毒是大气压等离子体射流（APPJ）在医学应用领域中的主要方向之一。近些年来研究结果表明APPJ内活性粒子尤其是含氧活性粒子是其能够灭菌消毒的主要原因，但由于缺少先进的等离子体诊断技术，各个活性粒子在杀灭病菌中具体贡献权重仍然不清楚。本项目将以一种锂电池驱动的便携式等离子体射流装置作为等离子体源，采用激光诱导荧光、腔衰荡光谱、吸收光谱等诊断方法，对等离子体射流内不同活性粒子如OH、O、O3、NO、NO2等进行定量研究，获得这些活性粒子的绝对密度大小及其空间分布，并研究控制这些活性粒子密度的方法策略及产生机制。然后结合定量的灭菌实验，对三种不同类型的病菌进行灭活处理，探索出不同活性粒子在灭菌过程中贡献权重大小，并根据结果进一步优化等离子体射流工作参数，从而使等离子体射流在灭菌中具备应有的尺和度，加快等离子体射流在实际灭菌消毒中的应用。

大气压低温等离子体射流在生物医学中得到越来越多的关注，近些年来研究结果表明等离子体内活性粒子尤其是含氧活性粒子是其能够灭菌消毒的主要原因，但由于缺少先进的等离子体诊断技术，各个活性粒子在杀灭病菌中具体贡献权重仍然不清楚。本项目通过激光诱导荧光法（LIF），吸收光谱法，傅里叶变换红外光谱法（FTIR）对大气压低温等离子体射流内关键活性粒子进行了全面的，清晰的诊断研究。对各个活性粒子尤其是OH自由基，O原子等提出了不同控制方法。结合灭菌实验表明，OH自由基及O3在大气压等离子体灭菌中起到关键作用。本项目重点研究并揭示了大气压等离子体内关键活性粒子的生成机制，传输路径和调控方法，为大气压等离子体射流自身机理研究及在等离子体生物医学研究中提供了可靠的依据和参考。