亚麻木酚素合成基因Lus10005950的克隆与功能分析

Lignan, an important functional sanitarian substance, has significant role in anti-cancer and slowing down a series of chronic diseases, etc. Lignan is the hot spot of medicine but less research in plant territory. Flax seed is rich in lignan but there are differences among different varieties. Main cultural varieties with high lignan content are very scarce in current flax production, and there are also not available genes for lignan synthesis in breeding. Therefore, excavating deeply the key genes of flax lignan synthesis and using them in molecular improvement of high lignan could accelerate the progress of high lignan breeding in flax, and increase the additional values of flax products.. A candidate gene Lus10005950 of lignan synthesis has been identified through the conjoint analysis of multi-environments and multi-omics by our research team. This project plans to clone Lus10005950 based on previous research basis. Targeted editing of Lus10005950 will be performed by using the CRISPR/Cas9 technology, and its functions under lignan synthesis in flax will be verified by combining the overexpression. The RNA-seq will be used to analyze its regulatory network. Functional molecular marker will be developed to perform the assisted selection for high lignan content. This project is aimed at resolving the function of Lus10005950 in lignan synthesis of flax, and obtaining the new high lignan materials of flax. This project could establish an important basis for breeding the new high lignan varieties of flax.

木酚素是一种重要的功能保健物质，在抗癌和减缓一系列慢性疾病等方面具有显著功效，是医学研究的热点，但在植物中研究极少。亚麻种子中富含木酚素，但品种间有所差异，生产中缺乏高木酚素主栽品种，育种中没有可利用的木酚素合成基因。因此，深入挖掘亚麻木酚素合成的关键基因，利用其进行高木酚素分子改良，可加快高木酚素亚麻育种进程，提高亚麻产品的附加值。. 本团队前期通过多环境、多组学联合分析，鉴定出一个木酚素合成候选基因Lus10005950。本项目拟在前期研究的基础上，对Lus10005950进行克隆；利用CRISPR/Cas9定点编辑技术，结合过量表达验证其在亚麻木酚素合成过程中的功能；通过RNA-seq分析其调控网络；开发功能分子标记，进行高木酚素辅助选择。本项目旨在解析Lus10005950在亚麻木酚素合成过程中的功能，获得高木酚素亚麻新材料，为培育高木酚素亚麻新品种奠定基础。

植物木酚素是重要的功能保健性成分，亚麻籽中木酚素含量极高，但亚麻的木酚素性状国内外研究较少，可利用的亚麻木酚素合成相关基因更是有限。项目前期利用GWAS，BSA和RNA-seq相结合的方式筛选到亚麻木酚素合成关键调控基因Lus10005950，在此基础上本项目完成了Lus10005950基因的克隆、载体构建及亚细胞定位。通过转基因功能验证，初步证实Lus10005950正向调控种子中木酚素合成，同时还负向调控茎秆中G型木质素单体的积累，且并不影响过表达植株抗倒伏性。本项目发现Lus10005950的功能具有多效性，为今后深入研究其协同调控亚麻纤维品质和营养品质的机制及培育亚麻新品种奠定坚实的基础。