高散射体激光双轴差动共焦显微拉曼光谱原位成像方法与技术

Raman scattering for in situ chromatography measurement in a high scattering object is the major measurement problem in the frontiers foundation research works such as biomedicine, material engineering, physical chemistry, high-end manufacturing and imaging measurement. To solve this common problem, laser dual-axes differential confocal Raman scattering for in situ tomography imaging method and technology in a high scattering object are proposed in the application project, which have anti-interference capability for stray light and high chromatography capability. Based on the dual- axes differential confocal detection technology proposed by us, the proposed method uses the reflected light from the sample abandoned by confocal-Raman spectra measurement system to form dual- axes differential confocal measurement system. This system can achieve the chromatography measurement for micro-region geometrical parameters and the triggering sampling of Raman-spectra at the focus, and thereby achieving the micro-region geometrical positions and Raman spectra for in situ high resolution chromatography measurement. The works include Raman spectra scattering theory for performance multi-parameters measurement, dual-axes differential confocal measurement method, and the dual-axes differential confocal Raman spectra imaging method, the establishment of corresponding system, the experimental verification of the imaging method, and the decoupling and characterization for multi- performance parameters, and so on. The technique specifications are the axial resolution ≤ 5 nm, lateral resolution ≤ 5 nm, detection depth >600?m and spectrum resolution ≤1 wave number. This project will provide a new micro-region Raman scattering for in situ chromatography measurement method in a high scattering object, the work is of significance in science and academic researches.

高散射体微区拉曼光谱的原位层析检测是目前生物医学、材料工程、物理化学、高端制造、成像检测等前沿基础研究中面临的重大测试问题，针对此共性问题，本项目提出研究具有抗杂散光能力和高层析能力的高散射体激光双轴差动共焦显微拉曼光谱原位成像方法与技术，该方法基于自行发明的双轴差动共焦探测技术，利用共焦拉曼光谱探测系统中遗弃的样品反射光构建双轴差动共焦检测系统，在实现微区几何参数层析检测的同时，还进行拉曼光谱的焦点触发采样，继而实现微区几何参数信息与拉曼光谱信息的高分辨原位层析检测。研究包括:多性能参数测量的拉曼光谱散射基础、双轴差动共焦检测方法、双轴差动共焦显微拉曼光谱成像方法、系统构建与实验验证、多性能参数解耦与表征等。技术指标：轴向分辨力≤5纳米，横向分辨力≤3微米，探测深度＞600微米，光谱分辨率≤1波数等。项目拟为高散射体微区拉曼光谱的原位层析检测提供全新的方法，具有重要的科学意义和学术价值。

高散射体微区拉曼光谱的原位层析检测是目前生物医学、材料工程、物理化学、高端制造、成像检测等前沿基础研究中面临的重大测试问题，针对此共性问题，本项目研究了具有抗杂散光能力和高层析能力的高散射体激光双轴差动共焦显微拉曼光谱原位成像方法与技术，该方法基于自行发明的双轴差动共焦探测技术，利用共焦拉曼光谱探测系统中遗弃的样品反射光构建双轴差动共焦检测系统，在实现微区几何参数层析检测的同时，还进行了拉曼光谱的焦点触发采样，继而实现微区几何参数信息与拉曼光谱信息的高分辨原位层析检测。研究包括:多性能参数测量的拉曼光谱散射基础、双轴差动共焦检测方法、双轴差动共焦显微拉曼光谱成像方法、系统构建与实验验证、多性能参数解耦与表征等。技术指标：轴向分辨力≤5 纳米，横向分辨力≤3 微米，探测深度＞600 微米，光谱分辨率≤1 波数等。项目共发表期刊论文 17 篇，申请发明专利 12 项，研制了激光双轴差动共焦显微拉曼光谱成像仪器，为高散射体微区拉曼光谱的原位层析检测提供全新的方法，具有重要的科学意义和学术价值。