新型含"界层结构"仿生一体化支架与人脐带MSCs工程化制备关节骨软骨复合组织研究

At present the articular cartilage of the composite tissue engineering development strategy extremely leads to quality defects of tissue regeneration and poor integration between graft and host, which is difficult to ensure its long-term effectiveness. Among them, Lack of layer structure between bone and cartilage and the source of the wide,stability and ideal seed cells are the most important reason and technical bottleneck. Therefore, under with the guidance of the new concept that engineering "Transparent cartilage- Boundary layer structure-subchondral bone"of bionic integration construction"put forward early, by making the latest research results at home and abroad use for reference, the stability between human umbilical cord mesenchymal stem cells and human bone marrow derived mesenchymal stem cells,the research of nano-HA/CPPf/PLLA bionic integration stents containing collagen coating,the external reconstruction of bone and cartilage tissue composite of "layer structure", the optimization in fostering technology maturation,and the large animal experimental study of long-term effects and composition outcome in renewable function, the study provide the optimal selection of most suitable for seed cells,the preparation of"layer structure" bionic integration stents and the construction in developing new technology method and fostering maturation,the the necessity and important function in the engineering construction of " Boundary layer structure " ,and the treatment joint osteochondral defects by engineering "bionic integration building" composite tissue technology regeneration "containing " Boundary layer structure " with necessary theoretical support, experimental basis and technical parameters.

目前复合组织工程策略研制的关节骨软骨，极易导致再生组织质量缺陷、移植物与宿主整合欠佳，难以确保其长期有效性。其中缺少骨与软骨之间的"界层结构"及来源广泛、稳定性良好的理想种子细胞，是其最重要原因和技术瓶颈。据此，本项目拟在前期提出的"工程化"透明软骨－界层结构－软骨下骨"仿生一体化构建"新理念指导下，通过人脐带MSCs与骨髓MSCs增殖分化稳定性比较、含"界层结构"胶原涂层nano-HA/CPPf/PLLA仿生一体化支架研制、含"界层结构"工程化骨软骨体外构建与熟化培育技术优化及其再生修复作用的长期效果与组分转归的大动物实验研究，为其最适宜种子细胞的优化选择、含"界层结构"仿生一体化支架制备及其体外构建与熟化培育新技术方法的建立、"界面结构"在其工程化构建中的必要性和重要作用，以及含"界层结构"仿生一体化构建的复合组织工程技术再生治疗关节骨软骨缺损，提供必要的理论支持、实验依据及技术参数。

目前复合组织工程策略研制的关节骨软骨，极易导致再生组织质量缺陷、移植物与宿主整合欠佳，难以确保其长期有效性。其中缺少骨与软骨之间的"界层结构"及来源广泛、稳定性良好的理想种子细胞，是其最重要原因和技术瓶颈。据此，本项目在前期提出的"工程化"透明软骨－界层结构－软骨下骨"仿生一体化构建"新理念指导下，通过人脐带MSCs与骨髓MSCs增殖分化稳定性比较、含"界层结构"nano-HA/CPPf/PLLA仿生一体化支架研制、含"界层结构"工程化骨软骨体外构建与熟化培育技术建立及其再生修复作用的大动物实验研究，为其最适宜种子细胞的优化选择、含"界层结构"仿生一体化支架制备及其体外构建与熟化培育新技术方法的建立、"界面结构"在其工程化构建中的必要性，以及含"界层结构"仿生一体化构建的复合组织工程技术再生治疗关节骨软骨缺损，提供了一定的理论支持、实验依据及技术参数。