家蚕对重大病毒病（BmNPV）抗性基因定位及鉴定

The research on silkworm (Bombyx mori) major virus disease BmNPV has been continued for over 100 years since the discovery of its pathogen, and great achievements have been made on transmission pattern , genomic structure and gene functions of the virus. However, silkworm breeding for BmNPV-resistant strains has been unsuccessful for a long time due to lack of virus-resistant natural silkworm strains. Fortunately, in 1990 we found a silkworm mutant from the silkworm resources maintained in our country, which showed high resistance to BmNPV. Since then we have been working on the virus resistance mechanism of this mutant strain for more than 20 years, and found that the resistance is controlled by single dominant gene. This is supported by the findings in the case of apple tree moth by Asser-Kaise, as well as by our experimental breeding practice. Unfortunately, the virus-resistant genes in silkworms and other insects have been largely unknown. To search for these genes, in this study we plan to use the SLAF-seq technique and other molecular biological technologies to accurately locate and identify the BmNPV-resistant gene in the silkworm mutant strain. Our work will solve the silkworm resistance mechanism to BmNPV and lay solid foundation for successful breeding of BmNPV-resistant silkworm strains, which will greatly benefit the sericulture in our country and contribute to the worldwide sericulture science, as well as the utilization of baculovirus as biological pesticides.

家蚕BmNPV病毒自发现后，相继研究已经有100多年，在病毒传染方式、病毒基因组及基因功能等方面取得很多研究成果。长期以来由于未发现高抗材料，致使家蚕抗病育种难以发展。1990年，申请者在我国保存的家蚕资源中，发现一个对BmNPV高抗的突变品系，经过其后20多年的不断研究，阐明了该品系抗性遗传规律为显性单基因控制，其结论也被Asser-Kaise等 研究苹果树蛾所佐证，同时也被抗病育种实践所证实。但家蚕以及其它昆虫的抗性基因至今未获得，因此，本研究旨在采用SLAF-seq技术以及其它分子生物学手段定位和鉴定抗性基因，彻底解决蚕业生产中家蚕对BmNPV抗性的难题，为世界蚕业科学以及杆状病毒作为杀虫剂做出贡献。

家蚕是一种重要的经济昆虫，家蚕BmNPV病对世界蚕业生产危害巨大。对BmNPV相继研究已经有100多年，虽然在病毒传染方式、病毒基因组及基因功能等方面取得了很多研究成果，但是由于未发现高抗材料，致使家蚕抗病育种难以发展。本项目利用我国家蚕资源库中筛选到的一个独有的对BmNPV完全抵抗的品系，通过生物学测试和分子水平鉴定，证实完全抵抗BmNPV病毒。通过构建遗传作图群体，高通量测序构建遗传图谱，将该基因定位在家蚕第27号染色体，通过分子生物学技术确定了抗性候选基因，并验证了部分基因的功能。获得紧密连锁的分子Marker,这个Marker对开展家蚕分子育种有很大帮助。采用HiC和三代技术完成了对抗性品系家蚕基因组的从头测序，并继续进行抗性基因的鉴定工作。同时开展了家蚕与病毒相互作用以及毒理学研究。