蒙古族肥胖儿童肠道菌群结构的变化及其对炎症反应的影响

Obesity of children has become a global problem affecting human health, but its pathogenesis is not fully clear. The pathogenesis research will provide scientific basis for the prevention and treatment of obesity in children. In this study, according to the theory “obesity induced chronic inflammation is closely associated with changes of intestinal flora” and the basis of body weight and lipid metabolism was regulated by intestinal flora in our previous animal study, 7 to 13-year-old Mongolian children in the eastern and western Inner Mongolia region will be recruited by the physical examination through random cluster sampling method.In WGOC as the standard for BMI screening, normal weight,overweight and obese Mongolian children are selected for different groups. collection of fresh stool samples, analysis the structure of the intestinal flora and the content of dominated flora by using PCR-DGGE and real-time PCR technology are undertaken. Determination of blood lipids and inflammatory related biomarkers. In addition, intestinal flora of high-fat fed male SD rats are disturbed by antibiotics to further investigate the effect of the intestinal flora on inflammatory responses, and mechanism involving AMPK pathway associated inflammatory response are revealed by using SCFAs treatment on intestinal cells.From the perspective of the gut microbiology to explore the pathogenesis mechanisms of Mongolian children with overweight and obesity. The research results not only will provide first-hand information of prevention and control Mongolian children with simple obesity, but will also provide new ideas for the study of obesity in children.

儿童肥胖已成为影响人类健康的全球性问题，但其具体发病机制不详。对其病因进行深入研究，可为儿童肥胖以及成年期肥胖的防治提供科学依据。本研究拟结合肠道菌群与肥胖及炎性反应发生密切相关的理论，在课题组前期研究肠道菌群调节动物体重和血脂代谢的基础上，采用随机整群抽样的方法抽取内蒙古东西部地区7～13岁蒙古族儿童，通过体格检查，以WGOC为标准进行BMI筛查，选择正常体重、超重和肥胖的蒙古族儿童为研究对象。利用PCR-DGGE及real-time PCR技术，分析新鲜粪便中肠道菌群结构及优势菌群的含量，测定血脂及炎性反应相关指标，再通过抗生素干预雄性SD大鼠肠道菌群，进一步探讨肠道菌群对炎性反应的影响，并利用SCFAs处理细胞揭示炎性反应相关的AMPK通路。从肠道微生物学角度分析蒙古族儿童肥胖发生机制，该研究结果不仅为蒙古族儿童肥胖的防控工作获得第一手资料，同时也为我国儿童肥胖的研究提供新的思路

儿童肥胖已成为影响人类健康的全球性问题，但其具体发病机制不详。对其病因进行深入研究，可为儿童肥胖以及成年期肥胖的防治提供科学依据。本研究采用随机整群抽样的方法抽取内蒙古东西部地区7～13岁蒙古族儿童，通过体格检查，以WGOC为标准进行BMI筛查，选择正常体重、超重和肥胖的蒙古族儿童为研究对象，分析新鲜粪便中肠道菌群结构及优势菌群的含量，测定血脂及炎性反应相关指标，再通过抗生素干预雄性SD大鼠肠道菌群，进一步探讨肠道菌群对炎性反应的影响，并利用SCFAs处理细胞揭示炎性反应相关的AMPK通路。结果显示：同一地区蒙古族正常体重、超重和肥胖儿童肠道菌群结构有显著差异，不同BMI蒙古族儿童肠道菌群多样性丰度具有显著性差异，体重正常组儿童肠道菌群的多样性和丰富度均高于肥胖组以及超重组；内蒙古东西部地区进行比较，不同BMI蒙古族儿童肠道菌群数量呈现肥胖组脆弱拟杆菌、双歧杆菌属、乳杆菌属的数量均低于正常体重组，揭示肠道菌群与肥胖之间具有一定的相关性；东西部地区蒙古族儿童肠道菌群和炎症因子的相关性显示，双歧杆菌属与IL-10呈正相关，与TNF-α呈负相关，乳酸杆菌属与IL-10呈正相关，与TNF-α、IL-1β呈负相关，揭示肥胖是一种慢性低度炎症反应；抗生素干预2周后，显著降低了正常大鼠肠道菌群的多样性，肥胖大鼠肠道菌群多样性进一步降低，但肥胖大鼠的机体炎症程度有所缓解，揭示肠道菌群与肥胖及炎症反应的发生密切相关；短链脂肪酸使磷酸化pAMPK含量下降，AMPK通路被抑制，造成细胞内TC、TG含量降低，说明短链脂肪酸通过抑制AMPK激活，而促进脂肪的分解，但此研究结果仍需要深入探讨。本研究从肠道微生物学角度分析蒙古族儿童肥胖发生机制，该研究结果不仅为蒙古族儿童肥胖的防控工作获得第一手资料，同时也为我国儿童肥胖的研究提供新的思路。