聚集诱导发光的金属纳米簇的可控制备、机制探讨和生物传感应用

Design and development of luminescent materials with aggregation-induced emission are of great significance in practical application of biosensing, bioimaging and optoelectronic devices. In this project, we intend to develop controllable preparation of metal nanocluster with aggregation-induced emission, investigate its underlying luminescence mechanism, and apply these nanoclusters to biosensing. Key points of the project include the following four aspects: controllable synthesis of metal (gold, silver and copper) nanoclusters with significant aggregation induced luminescence properties, exploration of aggregation induced luminescence mechanism of these metal nanoclusters, design of detection strategies based on aggregation-caused emission signal transduction by external stimuli, and development of the biosensors mediated by photoinduced electron transfer mechanism. The goals of this project are to achieve controllable preparation of a variety of metal nanoclusters with significant aggregation induced emission property, to reveal the intrinsic nature and the potential rules for this specific AIE phenomenon, to prepare various metal nanoclusters with different chemical compositions, different colors and different ligands on surface, and to functionalize metal nanoclusters with different functionals for AIE purpose. Based on surface-functionalized metal nanoclusters, this project is devoted to design and develop the quantitative detection and real-time monitoring methods for different biological molecules and biological processes through the aggregation processes induced by a variety of approaches including weak interaction, metal coordination, dynamic covalent reactions, click reactions and enzymatic reactions. The focus will be on establishment of detection methods for several enzymes activity, and their evaluation of these assays in cells or in vivo.

聚集诱导发光材料在生物传感、生物成像和光电器件领域具有重要的应用价值。本项目拟开展具有聚集诱导发光性质的金属纳米簇的可控制备、机制探讨和生物传感应用研究，通过具有该性能的金属纳米簇的可控合成、聚集发光机制探讨、基于外在刺激引起聚集诱导发光信号传导机制的检测策略的设计和基于光诱导电子转移信号传导机制的生物传感器的开发与应用等四个方面内容探索具有显著聚集诱导发光性质的金属纳米簇的可控制备方法，探寻聚集发光的内在机制和潜在规律，制备不同化学组成、不同发光颜色、不同表面配体的金属纳米簇，设计和合成具有显著聚集诱导发光性能且易于表面功能化的金属纳米簇。基于表面功能化金属纳米簇，通过弱作用力、金属配位、动态共价反应、点击反应和酶促反应等不同反应体系触发的聚集过程，设计和发展针对不同生物分子和生物过程的定量检测和实时监测方法，重点开发针对多种生物酶活性的检测方法，并评估其在细胞或活体内的传感性能。

本项目制备了不同化学组成、不同发光颜色、不同表面配体的金属纳米簇，并设计和合成了具有显著聚集诱导发光性能且易于表面功能化的金属纳米簇。探索了具有显著聚集诱导发光性质的金属纳米簇的可控制备方法，探寻了金属纳米簇聚集诱导发光的内在机制和潜在规律。基于表面功能化金属纳米簇，通过不同反应体系触发的聚集过程，设计和发展了针对不同生物分子和生物过程的定量检测和实时监测方法，并评估了其在细胞或活体内的传感性能。本项目的完成实现了具有AIE性质的金属纳米簇的可控制备，揭示了影响金属纳米簇AIE性能的关键因素。同时将聚集诱导发光过程和光诱导电子转移过程用于生物酶活性的检测策略中，通过两种不同信号传导机制的检测策略的建立，发展了一系列高灵敏的生物酶活性检测方法，并用于细胞和活体内的生物学评估研究，为构建相关疾病早期检测和治疗的多功能纳米器件提供了基础和借鉴。