基于超亲水微井界面的痕量循环miRNA检测新原理和新方法

Circulating microRNAs （miRNAs）play a very important role in development and progression of tumor.The detection of circulating miRNAs is critical for early detection of cancers, treatment monitoring, and evaluation of its postsurgical recurrence. Due to its low abundance, the main challenge in detecting miRNAs is to improve the sensitivity and reduce the amount of specimen. In this proposal, three-dimensional superhydrophobic porous nanocoatings were fabricated and superhydrophilic microwell was made in the as-prepared coatings. Based on the superwetting property of interface, the enrichment of circulating miRNAs occurs in the superhydrophilic microwell. On the basis of the enrichment behavior, the highly sensitive detection of miRNA can be achieved. Moreover, superhydrophilic microwell patterns will be designed for the high-throughout detection of miRNAs. This research offers an effective means for preparing biosensor interfaces with special wettability and provides a new approach for the effective detection of circulating microRNAs.

循环microRNA（miRNA） 在人体肿瘤发生和发展中发挥着重要的作用。循环miRNA的检测对于癌症的早期诊断、疗效评估和复发预测都具有重要的临床意义。由于循环miRNA在体液或血清中数量极少，如何提升纳米生物传感器检测灵敏度，减少检测样品用量，是目前面临的挑战性问题。本课题基于超疏水三维多孔纳米界面材料，在其表面构筑超亲水微井，合理利用纳米界面材料的超浸润性质，以肝癌相关循环miRNA为研究模型，探究传感界面浸润性质对miRNA分子富集行为的影响；在超亲水微井传感界面实现miRNA分子的高灵敏检测；构筑超亲水微井阵列，实现多组分肿瘤标志物的联合检测。本项目的开展为构筑新型传感界面提供新思路，为循环miRNA的高效灵敏检测提供了新方法。

MicroRNA作为肿瘤的一种新型标志物逐渐得到了重视。MicroRNA的检测对于癌症的早期诊断、疗效评估和复发预测都具有重要的临床意义。由于MiRNA在体液或血清中数量极少，如何提升纳米生物传感器检测灵敏度，减少检测样品用量，是目前面临的挑战性问题。本课题基于三维多孔纳米界面，在超疏水表面构筑超亲水微井，合理利用纳米界面材料的超浸润性质，研究miRNA等生物标记分子在传感界面的富集行为，实现了miRNA分子、蛋白质等分析的高灵敏检测；构筑超亲水微井阵列，同时实现生物标志物的富集与检测。本项目的开展为构筑新型传感界面提供新思路，为生物标记物分子的高效灵敏检测提供了新方法。