腹泻型肠易激综合征中医方-证-肠道微生态的宏基因组学研究

IBS-D is a common and difficult to treat， study Syndromes of TCM is the key to the effectiveness ,This study is based on the prophase molecular biology and clinical research, using metagenomic technology of traditional Chinese medicine (Tong xie yao fang, Shen ling bai zhu san ) intervention IBS-D of TCM syndrome (liver depression and spleen vacuity syndrome, spleen vacuity and damp encumbrance syndrome) were studied before and after the change of intestinal microecology, to reveal the IBS-D in different syndromes of metagenomics differences in traditional Chinese medicine based on syndrome differentiation achieved the effect of metagenomic basis and expression various types of related signaling pathways and key genes, designed to reveal the IBS-D with different syndromes metagenomic differences, the Chinese medicine diagnosis and treatment to be effective metagenomic basis to obtain each permit differential metagenomic expression，a preliminary metagenomic basis for IBS-D micro- syndrome differentiation

腹泻型肠易激综合征是多发病、难治病之一，其中医证候研究是中医辨证论治取得有效性的关键，本研究在前期分子生物学及临床研究的基础上，采用宏基因组学技术对中药（痛泻要方、参苓白术散）干预腹泻型肠易激综合征中医证候（肝郁脾虚证、脾虚湿阻证）前后的肠道微生态学变化进行研究，旨在揭示腹泻型肠易激综合征不同证型的宏基因组学差异、中医药辨证论治取得效果的宏基因组学依据以及获得各证型相关信号通路及关键基因的表达，达到以方测证，方证互验，从而为腹泻型肠易激综合征中医微观辩证奠定初步的肠道微生态宏基因组学基础。

本研究在前期分子生物学及临床研究的基础上，通过16s扩增子和宏基因组测序分析腹泻型肠易激综合征患者中，肝郁脾虚证和脾虚湿盛证患者在中药治疗前后肠道微生物菌群种类和丰度的变化趋势，发现不同证型治疗前后微生态宏基因组学表达的差异，中医证候与肠道微生物之间存在方-证-肠道微生态宏基因组学差异表达的效应关系，初步揭示中医传统辨证论治具有微观物质基础。本研究以方测证，方证互验，为该病的微观辨证初步奠定了宏基因组学基础。我们在研究中发现不同证型在治疗前后的肠道宏基因组学改变与代谢组学、胆汁酸代谢关系密切，故扩大了研究方向和范围，增加了相关代谢组学和胆汁酸代谢的研究，以便深入研究腹泻型肠易激综合征中医方-证-肠道微生态的微观物质基础及相关机制。