氯虫苯甲酰胺高效降解菌株GW13降解基因的克隆及功能研究

Chlorantraniliprole is a widely used pesticide and long residual in soil. chlorantraniliprole is considered as a persistent organic pollutant in soil. The residual chlorantraniliprole in soil not only directly pollute the environment but also severely influence the agricultural products safety. In our previous studies, a highly efficient chlorantraniliprole degradation strain GW13 was separated from soil and identified as Pseudomonas putida. However the degradation characteristics and mechanism is still unknow. In this study, we aimed to 1) develop the degradation characteristics of strain GW13; 2) construct genome library of GW13 and clone the key genes of chlorantraniliprole degradation; 3) study on the function of key genes of chlorantraniliprole degradation. Base on the study, chlorantraniliprole degradation characteristics of GW13 will be definited, chlorantraniliprole degradation pathway of GW13 will be derived and the function of key gene of chlorantraniliprole degradation will be described. This research will be helpful to deeply understand the metabolic process of chlorantraniliprole in environment, and provide theoretical basis for the controlling of chlorantraniliprole residue.

氯虫苯甲酰胺是一种应用广泛的杀虫剂，但在土壤中的残留期较长，被认为是持久性有机污染物，对环境和农产品质量存在安全隐患。前期研究中，申请人从农田土壤中分离获得一株氯虫苯甲酰胺高效降解菌株GW13，经鉴定为恶臭假单胞菌（Pseudomonas putida），但其降解特性及机理尚不明确。本项目拟应用分子生物学与分析化学的理论与方法，1）研究氯虫苯甲酰胺高效降解菌株GW13的降解特性；2）构建菌株GW13基因组文库及克隆降解氯虫苯甲酰胺关键基因；3）研究菌株GW13降解氯虫苯甲酰胺关键基因的功能。通过以上研究，明确氯虫苯甲酰胺高效降解菌株GW13的降解特性，推导菌株GW13降解氯虫苯甲酰胺的降解途径，阐明菌株GW13降解氯虫苯甲酰胺关键基因的功能。研究结果有助于深入解析GW13对氯虫苯甲酰胺在环境中的降解机理，为治理氯虫苯甲酰胺残留污染提供理论依据。