氨甲酰转移酶Asm21在安丝菌素生物合成中的催化二重性
基本信息
结项摘要
前期研究，从橙色束丝放线菌的发酵产物中分离到安丝菌素酰胺(N-氨甲酰基)糖苷类化合物；分析安丝菌素生物合成基因簇，只发现一个编码氨甲酰基转移酶的基因asm21；通过基因失活证明其功能是催化C-7位羟基的氨甲酰化；鉴于这三点推测Asm21除催化C-7位羟基的氨甲酰化外,还可能催化安丝菌素葡萄糖苷糖基的氨甲酰化，具有催化的二重性。本研究拟通过基因敲除、回补和饲喂实验，体内验证Asm21的二重催化功能；通过asm21的克隆和异源表达获得可溶性蛋白,以asm21突变株产生的19-chloroproansamitocin和安丝菌素酰胺N-糖苷为底物,体外研究Asm21的双重催化功能。同时考察Asm21不同结构域对成环反应的影响，并尝试Asm21对其他安莎类抗生素的氨甲酰基化。由于氨甲酰基往往是重要的活性基团，Asm21催化功能的揭示将为抗生素的组合生物合成改造提供新基因资源。
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数据更新时间：2023-05-31
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赵沛基的其他基金
相似国自然基金
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