细胞融合对植物基因组DNA结构影响的研究

The purpose of this projecT was to further elucidate the molecular nature of genomic effects of cytomixis on pollen mother cells during meiosis, which we had studied at the chromosomal level since 1980s under the continues financial support from NSFC. By using RAPD analysis we could dectected changes in genomic DNA sequences that specifically occurred at synizesis of meiotic prophase I. Sequence analysis revealed that one of these changes was caused by insertion of an LTR-retrotransposon that was closely related to the delfamilyof retrotranposon identified in Lilium henryi. This is interesting because it is the first report showing that retrotansposon can be activated specifically at meiotic synizesis. The coincidence of cytomixis and the activation of this retrotransposon suggests that retrotransposition might be caused cytomixis.

拟采用DNA 指纹技术（DNAfingerptint)、序列分析技术和染色体原子杂交等现代分子生物Х椒ǘ?染色质穿壁前后基因组DNA结构发生的改变、变化位点序列结构特征和在染色体上分布等进行深入研究，从分子水平探索细胞融合影响植物基因组DNA不稳定性的机理。该研坑兄谏罨嗣嵌韵赴诤舷窒笠糯庖宓娜鲜丁?..