基于微型板作为固载界面的流感病毒传感器研究

Biomimetic sensors based on molecular imprinted polymers as recognition elements has gradually become a hot topic in the field of biosensors.Surface molecular imprinting is an effective way of macromolecules imprinting. With the repeated outbreaks of flu, influenza virus was selected as the research object in the present project. Novel surface monolayer imprinting techniques for recognition and detection of influenza virus based on micro-plates are proposed. A basic scientific research of the imprinted methods and mechanism will be further investigated. The specific content includes: the oligomers and influenza virus may be spread on the substrate surface using electrodes and 96-well plates as interface board by spin coating technique, followed by polymerization to achieve surface monolayer imprinting. Novel biomimetic sensors of influenza virus will be constructed combining with highly sensitive analytic methods through signal transition of labeling technique. At the same time, the model of microscopic behaviors and adsorption mechanism in the imprinting process will be further researched. It would provide reference methods for the real-time monitoring and subtype of influenza virus and establish a theoretical and technical basis of imprinting technique with large size of the organism.

以分子印迹聚合物作为识别元件的仿生传感器已逐渐成为生物传感器领域的研究热点。表面分子印迹是生物大分子印迹的有效途径之一。本项目针对反复爆发的流感疫情，以流感病毒为研究对象，继传统的分析诊断技术之后，提出一种新型基于微型平板的表面单分子层印迹技术用于流感病毒的识别与检测，并对所构建的印迹方法及机理进行基础科学研究。具体内容包括：以电极和96孔酶联免疫板为固载界面，采用表面旋涂技术，将低聚物和流感病毒颗粒先后平铺于载体表面，继而进行聚合反应，实现表面单分子层印迹，利用标记技术进行信号的转变，结合高灵敏的分析方法构建新型流感病毒仿生传感器。同时进一步研究流感病毒印迹过程中的微观行为模式及吸附机理，为流感病毒的实时监测及亚型分类提供方法学参考，也为印迹技术在大粒径生物体中的应用奠定理论和技术基础。

以分子印迹聚合物作为识别元件的仿生传感器已逐渐成为生物传感器领域的研究热点。表面分子印迹是生物大分子印迹的有效途径之一。本项目主要围绕分子印迹技术及新型功能纳米材料展开，并将之应用于生物分子(流感病毒、蛋白质、等)的识别与检测。主要内容包括：以电极和96孔酶联免疫板为固载界面，以抗体或者表面单分子印迹层为识别元件，通过酶和功能化纳米材料的标记进行信号转换与放大，结合高灵敏的分析方法构建新型仿生传感器，从而实现生物大分子（蛋白质、流感病毒）等的识别与检测，并对所构建的传感技术及机理进行基础科学研究，为印迹技术在大粒径生物体中的应用奠定理论和技术基础。