脂类代谢重要基因ACBP在水稻脂类降解中的作用机制及功能研究

Acyl-CoA-binding proteins，ACBPs, are lipid transporters that function in lipid metabolism. In eudicots, besides the ability to bind acyl-CoA and phospholipids, ACBPs are also involved in the stress responses. Investigations on rice ACBP family show that OsACBP6 is unique due to the localization to the peroxisomes, an organelle closely related to lipid degradation. But other plant ACBP members are localized to the organelles function in lipid biosynthesis, such as cytosol, plasma membrane and endoplasmic reticulum. Therefore, this application is to investigate the function of OsACBP6 in rice. Wild type rice, T-DNA insertion mutant osacbp6, RNA interference lines and overexpression lines will be used as materials. By comparison of the phenotypes, proteomics, lipid profiles and pathways involved in lipid degradation, the function of OsACBP6 in lipid metabolism especially in lipid degradation will be elucidated. It will enrich the functional diversity of ACBP in plant and also benefit further understanding the lipid metabolism in monocots.

酰基辅酶A结合蛋白（acyl-CoA-binding proteins，ACBPs）是一类脂类载体蛋白。在双子叶植物中，ACBPs除了具有结合和运输脂类物质的功能，它们还参与了植物生长发育和胁迫反应。在前期对水稻ACBP家族的研究中，发现了一个有别于其他植物ACBP的成员OsACBP6，它定位于与脂类降解有密切联系的过氧化物酶体中。而其他的ACBP定位于与脂类合成相关的细胞器，如细胞质，细胞膜和内质网等。因此，本项目拟对OsACBP6在水稻中的具体功能进行研究。以野生型水稻、OsACBP6基因功能缺失突变体、表达下调的RNA干扰植株和过表达植株为研究材料，开展以下内容：1.比较表型和蛋白质水平的差异；2.分析脂类成分的组成；3.研究脂类降解相关的途径。从而揭示OsACBP6在脂类降解中的作用，为进一步了解植物ACBP的功能多样性奠定基础，同时为阐明单子叶植物脂类代谢途径提供支持。