构建具有信号放大功能的高灵敏，高便捷的中药材基因鉴别的电化学DNA传感器的研究

Because of genetic detection technology is of great application value in the study of traditional Chinese medicine identification. This topic will study on the construction of the function of signal amplification of high sensitivity, high convenient electrochemical DNA sensors for Chinese herb gene identification. Compared with method of molecular biology which are currently used in traditional Chinese herb genetic detection , this sensor can identify Chinese traditional herb gene sequencing directly through hybridization reaction, which has been proved to be a simple operation, as well as fast and cheap. Multi-ferrocenly compund labeled DNA probe and nanocomposites modified electrode together to build the sensor，which can provide greater electrochemical signals for hybridization sequencing detection, the sensitivity of the sensor and convenience is greatly increased compared with the traditional electrochemical DNA sensorwe will use the synthetic materials to build a varietu of detection model of electrochemical gene sensor and research on their characteristics in order to select a two kind of the application of the rared traditional chinese herb and its electrochemical gene identification sensor.

由于基因检测技术在中药鉴别研究中具有极其重要的应用价值。本课题将研究具有信号放大功能的高灵敏，高便捷的中药材基因鉴别的电化学DNA传感器。相较于目前应用于中药材基因检测的分子生物学方法，这种传感器能够通过DNA杂交反应对中药材基因进行直接测序鉴别，且具有操作简单，快速，高灵敏等优点。与传统电化学DNA传感器相比，新型多二茂铁基标记的DNA探针与纳米复合材料修饰的电极共同构建传感器，能够提供更大的电化学信号用于杂交测序检测，使得传感器的灵敏度和便捷性大大提高。我们将利用合成的材料构建多种检测模式的电化学基因传感器，并研究它们的特性，以筛选出一到两种的应用于名贵中药材（人参属药材及冬虫夏草）及其伪品的电化学基因鉴别传感器。

中医药是中国传统文化的瑰宝，而自古以来对中药材鉴定都是中药研究的重要课题。中药材基因鉴别的分子生物学技术，虽然其准确性高，但是操作繁琐，实验条件苛刻，检测仪器体积较大，通常检测只能在实验室完成，不能满足目前快速，廉价的现场中药材鉴别的要求。于是发展高效、快速、廉价的新型中药材DNA鉴别方法，对于中药鉴别的研究是一项很有意义的工作。本课题合成了一系列的多二茂铁基化合物用作电化学标示物标记在探针DNA上，并制备具有优良导电功能的修饰电极，共同用于构建具有信号放大功能的高灵敏，高便捷的中药材基因鉴别的电化学DNA传感器，并应用于名贵中药材（冬虫夏草等）及其伪品的基因鉴别，同时还构建一款能直接对癌细胞中的RNA检测的超高灵敏度的传感器。