治疗性干细胞穿过血脑屏障的细胞和分子生物学研究

Mutlipotential stem cells represent the important therapeutic resource for human neurodegenerative diseases. One of key concerns in the clinic is to understand how these stem cells transmigrate the blood brain barrier (BBB) and initiate the stem cell-based tissue repair. Chemokines are a superfamily of small, most secreted chemoattractant cytokines and generally involve in the cell mobility in both physical and pathological conditions. Here, we will establish human induced pluripotent stem (ips) cells and characterize the expression of chemokine and their receptors in these cells. Further, we will study the detailed roles of chemokine signaling in ips stem cells during the BBB-transmigration in vitro and in vivo, resulting in the identification of key chemokines that are required to recruit the transplanted stem cells.

)：多潜能性干细胞在神经系统疾病的治疗中具有重要的应用价值，但也在实际操作上面临着许多技术上的难题。其中的一个难点就是"治疗性干细胞如何穿过血脑屏障进入人的中枢神经系统中？"。 趋化因子是一组超家族的小分子量的分泌型蛋白，功能上调节免疫细胞穿透血脑屏障进入到人的中枢神经系统并诱导炎症的发生。最 近的研究发现神经干细胞也表达趋化因子受体并且依赖趋化因子信号进行细胞移动，表 明神经干细胞也可能通过趋化因子信号的调节来穿过中枢血脑屏障。本 研究将利用细胞和整体动物模型来探讨趋化因子信号在神经干细胞穿过中枢血脑屏障的关键作用机理，为 治疗性干细胞的发展极其在神经疾病的临床应用提供理论基础。