“呼吸型”金纳米囊泡的制备及在靶向智能诊疗一体化中应用研究

Cancer activatable theranostics involve the combination of medical diagnostics and therapeutic moieties to achieve an optimizing efficacy and safety of comprehensive regime, is an emerging interdiscipline that is paving the avenue towards the goal of personalized medicine. Based on our previous studies, we will design and prepare a RGD peptide-conjugated doxorubicin (DOX)-loaded “breathing” gold nanovesicles (RGD-CO2-GVs-DOX) for targeting cancer activatable theranostics. Upon laser irradiation, ultrastrong plasmonic coupling between adjacent gold nanoparticles in the vesicular membranes will enhance photoacoustic (PA) and ultrasonic (US) imaging. Meanwhile, both temperature increase and US stimulus will induce the release of CO2 gas, which also can enhance PA and US imaging. Light-triggered temperature raise of gold nanovesicles and US stimulus will dissociate the RGD-CO2-GVs-DOX to release the encapsulated DOX molecules. The as-prepared RGD-CO2-GVs-DOX platform can be used for enhanced PA/US dual-modal imaging guided chemo-photothermal synergistic therapy of U87MG glioblastoma cancer.

癌症智能诊疗通过联合医学诊断和治疗手段以达到具有最佳治疗效率和最安全的综合诊疗手段，其高度的多学科交叉正在为个体化治疗铺平道路。本项目拟在我们前期工作的基础上，设计和制备“呼吸型”金纳米囊泡（RGD-CO2-GVs-DOX）用于靶向智能肿瘤诊疗一体化。在激光光照作用下，利用金纳米囊泡中金纳米颗粒之间的表面等离子耦合效应来实现肿瘤的增强光声和超声成像，同时温度升高和超声作用下，CO2释放，产生气泡能增强光声和超声成像信号强度。激光照射引起的囊泡温度升高和超声刺激，囊泡结构破坏，快速释放其中包裹的药物。该“呼吸型”金纳米囊泡平台有望实现对神经胶质瘤（U87MG）增强的光声和超声双模式成像指导的靶向化疗和光热协同治疗。

癌症智能诊疗通过联合医学诊断和治疗手段以达到具有最佳治疗效率和最安全的综合诊疗手段，其高度的多学科交叉正在为个体化治疗铺平道路。本项目在我们前期工作的基础上，设计和制备装载 NH4HCO3和 DOX 的“呼吸型”金纳米囊泡（GVND）用于智能肿瘤诊疗一体化。在激光光照作用下，利用金纳米囊泡中金纳米颗粒之间的表面等离子耦合效应来实现肿瘤的增强光声和光热成像，同时囊泡温度升高，促使包裹的NH4HCO3分解释放CO2。释放的CO2与囊泡表面聚合物反应，改变了其表面亲疏水性，从而破坏囊泡结构，使得包裹的药物DOX“爆炸式”释放。该“呼吸型”金纳米囊泡平台有望实现对神经胶质瘤（U87MG）增强的光声和光热双模式成像指导的靶向化疗和光热协同治疗。在此基金资助下，我们以第一或通讯作者身份发表SCI论文25篇。同时，申请中国发明专利13项, 其中授权3项。