蓖麻毒素DNA应急疫苗研究

As potentially important toxins and biological agents, Ricin escalated into potential threat on national public security, which can not be ignored. Vaccination is one of the most effective means to prevent ricin poisoning, but there is no specific antidote for ricin, vaccine research has been in the laboratory and clinical research. According to the potential threat of ricin, establishing effectively rapid response mechanism and coping strategies is not only the urgent need for governments, but also for many scholars. The study based on the existing mouse scFv and RTB of the research, humanized antibody gene library construction and screening of specific anti-ricin human single chain Fv antibody, construct human ScFv gene encoding ricin DNA vaccine and investigate the immune efficacy of DNA vaccination in mice. A new emergency vaccine can improve the antibody production speed, to achieve effective protection dose and continued for several months in a few hours. The purpose of the study is to establish a rapid and feasible emergency vaccine development course, which can effectively deal with ricin, including other biological threats, also, improve our counter-terrorism and emergency response ability, which has very important meaning in both military affairs and society.

蓖麻毒素作为潜在重要毒素战剂和生物恐怖剂，对国家公共安全有着不容忽视的潜在威胁。接种疫苗是预防蓖麻毒素中毒最有效的手段之一，但目前蓖麻毒素无任何专用解毒剂，疫苗研究还一直处于实验室和临床研究阶段。针对蓖麻毒素的潜在威胁，建立有效的快速反应机制和应对策略，是各国政府众多学者所迫切需要解决的问题。本研究在课题组已有鼠源单链抗体基因和RTB基因基础上，通过构建人源化抗体基因库，筛选特异性抗蓖麻毒素人源单链抗体基因，制备成DNA疫苗，评价分析疫苗在小鼠体内的抗体产生效果。该新型DNA应急疫苗可快速提升抗体生产速度，在几小时内能达到有效防护剂量且持续数月。本研究旨在建立一种快速可行的应急疫苗研制路线，对有效应对蓖麻毒素等生物威胁，提高我国反恐和应急处突能力，具有十分重要的军事和社会意义。

蓖麻毒素作为潜在重要毒素战剂和生物恐怖剂，对国家公共安全有着不容忽视的潜在威胁。接种疫苗是预防蓖麻毒素中毒最有效的手段之一，但目前蓖麻毒素无任何专用解毒剂，疫苗研究还一直处于实验室和临床研究阶段。本研究在课题组已有鼠源单链抗体基因基础上，构建人源化抗体基因库，通过筛选Tomlinson I+J噬菌体抗体库，成功筛选得到全人源抗RT单链抗体JE11，制备鼠源和人源DNA疫苗，评价分析疫苗在小鼠体内外的抗体产生效果。该新型DNA应急疫苗可快速提升抗体生产速度，对有效应对蓖麻毒素等生物威胁，提高我国反恐和应急处突能力，具有十分重要的军事和社会意义。