电针足三里介导PKC调节IBS大鼠平滑肌细胞收缩蛋白的作用机制

The smooth muscle of colon plays a function of contraction, relaxation, synthesis and secretion in intestinal motility of irritable bowel syndrome (IBS). Protein kinase C (PKC) plays an important role in the regulation of smooth muscle contraction. On the basis of our previous studies on IBS smooth muscle cytoskeleton proteins by acupuncture, the law of colonic movement during the intestinal inflammatory response of IBS needs further explored. Using electroacupuncture (EA) therapy, targeting on contractile function and inflammatory mechanism of smooth muscle. The contraction mechanism of smooth muscle cells (actin polymerization and vimentin reorganization) is used as a target to observe the effect of acupuncture on contractile protein in smooth muscle cells of IBS rats. Meanwhile, taking the upstream signaling pathway protein kinase C (PKC) as a juncture, the applicant also aims to explore the correlation between smooth muscle cell phenotype transformation and inflammation. Thus, the mechanisms would further reveal the understanding of acupuncture effect and provide new experimental basis for clinical prevention and treatment on IBS.

在肠易激综合征（IBS）肠道蠕动中，结肠平滑肌发挥着收缩、舒张、合成和分泌的功能。蛋白激酶C（PKC）对平滑肌收缩功能具有重要调节作用。本课题在前期针刺调节IBS平滑肌细胞骨架蛋白功能机制研究的基础上，进一步探讨IBS肠道炎症反应过程中结肠运动规律；通过电针干预，以结肠平滑肌收缩功能和炎性机制为切入点，研究针刺对IBS大鼠平滑肌细胞表型和结肠炎症的调节作用；以平滑肌细胞收缩机制（肌动蛋白聚合和波形蛋白重组）为靶点，观察电针对IBS大鼠平滑肌细胞收缩蛋白的影响；以上游调控蛋白PKC为契机，探讨平滑肌细胞表型转化和炎症的相关性以及针刺的调节作用，进一步阐明针刺作用机制，为临床防治IBS提供新的实验依据。

在肠易激综合征（IBS）肠道蠕动中，结肠平滑肌发挥着收缩、舒张、合成和分泌的功能。蛋白激酶C（PKC）对平滑肌收缩功能具有重要调节作用。本课题在前期针刺调IBS平滑肌细胞骨架蛋白功能机制研究的基础上，进一步探讨IBS肠道炎症反应过程中结肠运动规律；通过电针干预，以结肠平滑肌收缩功能和炎性机制为切入点，研究针刺对IBS大鼠平滑肌细胞表型和结肠炎症的调节作用；以平滑肌细胞收缩机制（肌动蛋白聚合和波形蛋白重组）为靶点，观察电针对IBS大鼠平滑肌细胞收缩蛋白的影响；以上游调控蛋白PKC为契机，探讨平滑肌细胞表型转化和炎症的相关性以及针刺的调节作用，进一步阐明针刺作用机制，为临床防治IBS提供新的实验依据。