基于LIF/STAT3信号通路探讨补肾活血法改善子宫内膜容受性的分子机制

The incidence of infertility in women has gradually increased in recently years.Assisted reproductive technology (ART) is a important treatment to infertility. But the development of ART was restricted by the endometrial receptivity . Embryo implantation into the uterus is a critical step in the establishment of pregnancy and failure of this process is a major cause of infertility in women. It was effective to improve the endometrial receptivity with the traditional Chinese medicine. The study with the LIF/STAT3 signaling pathway to exlore the mechanism through animal experiment and cell experiment, explore the relationship between the LIF/STAT3 signaling pathway and the reinforcing kidney and activating blood circulation.Through the model of Super Ovulation in the rat , analysis the changes of protein and gene in the LIF/STAT3 signaling pathways,discussion the effective of the reinforcing kidney and activating blood circulation through the LIF/STAT3 signal pathway. Through the model of the siRNA silencing LIF/STAT3 signal pathway, analysis the of mechanism of reinforcing kidney and activating blood circulation with Western-blot and real-timePCR technology . We will explore the molecular mechanism of traditon chinese medicine on the ART in the reaserch.

辅助生殖技术（ART）是治疗不孕症的有效手段，目前子宫内膜容受性不良导致的低妊娠率制约ART的发展，中医药在“肾主生殖”理论的指导下改善子宫内膜容受性的疗效得到认可，但分子机制不明。我们在前期临床研究证明补肾活血法能改善子宫内膜容受性，在此基础上本课题首先复制大鼠超促排卵模型，以补肾活血法进行干预，通过免疫组化、western-blot及RT-PCR技术检测LIF/STAT3信号通路各分子的蛋白及基因改变，探讨LIF/STAT3信号通路与子宫内膜容受性的相关性。通过建立siRNA沉默STAT3基因干扰LIF/STAT3信号通路的大鼠子宫内膜细胞模型，应用western-blot及RT-PCR等技术检测LIF/STAT3信号通路各分子的变化，探讨补肾活血法的作用靶点，以阐明中医药改善子宫内膜容受性治疗不孕症的作用环节，为中医药在辅助生殖技术中的应用提供科学依据。

辅助生殖技术（ART）是治疗不孕症的有效手段，目前子宫内膜容受性不良导致的低妊娠率制约ART的发展，中医药在“肾主生殖”理论的指导下改善子宫内膜容受性的疗效得到认可，但分子机制不明。我们在前期临床研究证明补肾活血法能改善子宫内膜容受性，在此基础上本课题首先复制大鼠超促排卵模型，以补肾活血法进行干预，通过免疫组化、western-blot及RT-PCR技术检测LIF/STAT3信号通路各分子的蛋白及基因改变，探讨LIF/STAT3信号通路与子宫内膜容受性的相关性。通过本实验，证明补肾活血中药能够提高子宫内膜容受性的相关因子整合素β3、LIF、LIFR、gp130、STAT3、p-STAT3、VEGF的表达，从而改善子宫内膜容受性，提高受孕几率。通过建立siRNA沉默STAT3基因干扰LIF/STAT3信号通路的大鼠子宫内膜细胞模型，应用western-blot及RT-PCR等技术检测LIF/STAT3信号通路各分子的变化，通过实验证明补肾活血中药可以改善 LIF mRNA、STAT3 mRNA 、p-STAT3 mRNA经沉默后下降趋势。以Westem-blot进一步验证补肾活血中药改善沉默下降趋势，故补肾活血中药可以通过改善STAT3改善其相关信号通路，从而改善子宫内膜容受性。