海绵中共生蓝细菌来源天然产物的解明

Sponges are important sources of drug lead compounds. Some compounds isolated from Marine sponges could be produced by cyanobacterial Symbionts in Sponges. Studies on natural products originated from cyanobacterial symbionts in sponges are of great significance and urgency. Scientific evidences have demonstrated that fluorescence microscopy is the key to determine the occurrence of cyanobacterial Symbionts in sponge samples. The present project plans to employ the new machine fluorescence microscopy which is benefit for the determine the present of cyanobacterial. Cyanobacterial symbionts in sponges collected from the Lingshui sea area are cultured in our lab, which are then purified by traditional methods and identified by molecular means to obtain pure cultures. A library on Cyanobacterial Symbionts in Sponges with biological diversity will be established. Targets strains are selected by gene and chemical screening. OSMAC strategy is applied to induce chemical diversity of secondary metabolic. Modern techniques for natural products analysis and isolation are used to discover marine bioactive natural products with novel chemical structures. Determine the secondary metabolites originated from cyanobacterial symbionts in sponges by compareing the secondary metabolites gained from cyanobacterial symbionts in sponges with those produced from the sponges. This project planing to study natural products originated from cyanobacterial symbionts in sponges could explore new ideas to solve the problem of medicine source.

海绵是药物先导化合物的重要来源。一些活性物质可能是由与海绵共生的蓝细菌所产生。研究海绵中共生蓝细菌来源天然产物对海洋药物研发具有重要性和紧迫性。科学研究发现，荧光显微法是获得海绵中共生蓝细菌的关键。本项目拟采用蓝细菌分离新技术—荧光显微技术，对海南陵水海域海绵中的共生蓝细菌进行实验室分离，结合经典蓝藻纯化方法与分子鉴定技术，获得若干纯培养，建立多样性丰富的海绵共生蓝细菌库。通过基因与化学筛选，确定目标菌株。应用OSMAC策略诱导次级代谢产物多样性，综合集成现代天然产物分析纯化技术，发现若干具有新颖化学结构的海洋活性天然产物。对比海绵中次级代谢产物类型，确定海绵中次级代谢产物是由与海绵共生的蓝细菌所产生。本项目研究海绵中共生蓝细菌来源的天然产物，为解决药源问题探索新思路。

海绵是药物先导化合物的重要来源。一些活性物质可能是由与海绵共生的蓝细菌所产生。研究海绵中共生蓝细菌来源天然产物对海洋药物研发具有重要性和紧迫性。科学研究发现，荧光显微法是获得海绵中共生蓝细菌的关键。本项目从我国海南省三亚陵水湾海域共采集海绵15种，采用蓝细菌分离新技术—荧光显微技术，结合经典蓝藻纯化方法与分子鉴定技术，分离纯化蓝细菌7株，通过基因与化学筛选，获得有研究潜力的海洋蓝细菌2株。应用OSMAC策略诱导次级代谢产物多样性，综合集成现代天然产物分析纯化技术，从中发现29个单体化合物，其中6个为具有新颖化学结构的海洋天然产物。本项目研究海绵中共生蓝细菌来源的天然产物，为解决药源问题探索新思路。