基于磁性MOFs表面分子印迹的磁控高通量电化学传感检测食品中土霉素的研究

The problem of antibiotic abuse in the food industry is prominent. The excess of antibiotic residues in foods is not only affects the healthy development of animal husbandry, but also harmful to people's health. In order to solve the outstanding problems of molecular imprinting electrochemical sensor in the preparation and application in food safety detection (complex electrode modification, low flux detection), this work designs a new high throughput targeted electrochemical sensing analysis strategy for the determination of common antibiotic residual oxytetracycline in animal foods. This project focuses on the preparation of magnetic MOFs surface molecular imprinting materials and the use of magnetic electrode to build high-throughput electrochemical sensing analysis system, and the purpose of this work is to study the development of simple and quick, sensitive and reliable, high throughput targeted electrochemical sensing analysis method for the determination of oxytetracycline, and targeted sensing mechanism. The results of this research would broaden the application scope of molecular imprinting electrochemical sensing in food safety and environmental monitoring, which has far-reaching significance in speeding up the development of China's high throughput rapid on-site analysis technology of harmful substances in foods. In addition, this study could also provide technical support for the supervision and traceability of antibiotics exceeded products.

食品工业生产中抗生素滥用问题非常突出，食品中抗生素残留超标不但影响养殖业的健康发展，而且严重危害人体健康。本课题拟以动物性食品中常见抗生素残留土霉素为研究对象，针对分子印迹电化学传感器在制备和应用中存在的突出问题（电极修饰过程复杂、检测通量低），设计新型高通量靶向电化学传感分析策略，重点围绕磁性MOFs表面分子印迹材料的制备，以及利用磁控电极构建高通量电化学传感分析体系两个方面进行研究，旨在开发简便快捷、灵敏可靠且高通量的土霉素电化学传感分析方法，阐明土霉素靶向传感识别机理。本项目的研究成果将会拓展分子印迹电化学传感检测在食品安全和环境监测领域的应用，并对我国食品中有害物高通量现场快速检测技术的发展具有深远的意义。同时，此研究还可以为抗生素超标产品的监管和溯源提供技术支持。

本项目以食品中常见的农药残留与兽药残留为研究对象，针对分子印迹电化学传感器、分子印迹荧光传感器、适配体传感器在制备和应用中存在的突出问题，设计新型高通量靶向传感分析策略，重点围绕新型功能材料与识别材料的制备与应用、高通量传感分析体系的构建进行了相关研究，开发了一系列简便快捷、灵敏可靠的传感分析方法，应用于实际样品中目标物的检测，同时阐明了目标物靶向传感识别机理，这些研究成果为传感分析方法在食品安全快速检测领域的应用提供了有益的思路和方法，有利于拓展分子印迹光电化学传感检测在食品安全和环境监测领域的应用，对食品中有害物高通量现场快速检测技术的发展具有深远的意义。主要开展了以下研究工作：.1. 制备磁性MOFs表面分子印迹材料用于土霉素的特异性识别和吸附，配合磁控电极构建高通量电化学传感器的传感功能界面。所构建的磁性分子印迹电化学传感器成功应用于食品样品中土霉素的检测，克服了传统分子印迹电化学传感器在制备和应用过程中电极修饰过程复杂、检测通量低等突出问题。.2. 利用量子点独特的光学性质和分子印迹聚合物选择识别性强的优点，制备了量子点表面分子印迹材料作为乐果荧光识别探针，并借助微孔板，构建了基于量子点表面分子印迹材料的荧光传感器，该传感器实现了食品样品中乐果的高通量灵敏检测。.3. 利用重氮耦合方法将适配体通过共价键结合在电极表面，构建了可用于快速高效检测土霉素的适配体电化学传感器，该方法可以实现对土霉素稳定，快速，高灵敏度的测定，同时实现对牛奶样品中土霉素的快速检测，该方法克服了适配体电化学传感器适配体修饰不牢固，传感检测稳定性差等缺点。.4. 将纳米金和MOFs复合材料作为基底修饰在的石英晶片表面提高石英晶体微天平传感检测的灵敏度，随后将适配体通过共价键连接在石英晶片表面构建高灵敏度、高选择性的适配体石英晶体微天平传感器检测四环素，成功实现对牛奶中四环素的检测，拓宽了MOFs在石英晶体微天平传感器构建中的应用范围。