基于磁性分子印记聚合物的磁控电化学传感膜及临床标志物分析

An electrochemical sensing platform with high sensitivity and recognition will be fabricated based on our previous researches of nano-components, magnetic field directed self-assembly (MDSA) and biomimetic electrochemical nano-sensing film. For the templates, typical markers of clinical examination and diagnosis, the magnetic molecularly imprinted polymers (MMIPs) with electrochemical property have been designed. On the basis of MMIPs, the sensitive magnetic-controlled film which was non-immobilized above the electrode interface will be explored and built to establish a simple and fast clinical testing and diagnosis system with general applicability. Meanwhile, the nature and law of magnetic field induced self-assembly process will be known by investigating the composition, structure and characterization of sensing film at the molecular level. The structure–activity relationship will be examined and revealed between film structure and magnetism, electrical conductivity, electrocatalytic activity and molecular recognition characteristics. The project provides not only new notion, methodology for the molecular imprinting technology in electrochemical sensor study, but also valuable theoretical significance and practical application in the clinical marker analysis, study of physiological function, pathogenesis and mechanism of drug action.

本课题将在前期分子印记纳米元件、磁控自组装技术和仿生电聚合纳米传感膜研究和应用基础上，针对临床检验和诊断代表性标志物，设计具有电化学特性的磁性分子印记聚合物，探索研究电极界面非固化磁控性敏感膜，制备具有高灵敏和高识别特性的电化学传感器，建立一种简单、快速和具有普适性的临床检测与诊断体系。同时在分子水平探索传感膜的组成、结构和表征方法，认识磁控诱导自组装过程本质与规律，揭示其结构与磁性、电传导与电催化活性、分子识别特性的“构～效”关系。本项目不仅为分子印记技术用于电化学传感研究提供一种新思路、新方法，而且对临床标志物分析、生理功能、发病机理、药物作用机制等研究具有重要理论意义和广泛实用价值。

本课题在前期分子印记纳米元件、磁控自组装技术和仿生电聚合纳米传感膜研究和应用基础上，针对临床检验和诊断代表性标志物，设计出具有电化学特性的磁性分子印记聚合物，探索研究了电极界面非固化磁控性敏感膜，制备出具有高灵敏和高识别特性的电化学传感器，建立了一种简单、快速和具有普适性的临床检测与诊断体系，并成功用于多种基质样品中目标物的测定。同时在分子水平探索了传感膜的组成、结构和表征方法，认识了磁控诱导自组装过程本质与规律，揭示了其结构与磁性、电传导与电催化活性、分子识别特性的“构～效”关系。本项目不仅为分子印记技术用于电化学传感研究提供一种新思路、新方法，而且对临床标志物分析、生理功能、发病机理、药物作用机制等研究具有重要理论意义和广泛实用价值。.在扩展性研究中，基于临床标志物的广泛性，在分子印记电化学传感研究基础上，结合多样分子识别机制，扩展了纳米荧光与可见光传感等研究，并成功用于多种临床标志物分析，为临床诊疗和预后评价提供了更丰富的信息获取手段。