益气养阴祛瘀法调节干燥综合征颌下腺免疫微环境改善唾液分泌的作用研究

In the Sjogren's syndrome (SS), Abnormal local immune inflammatory microenvironment of the salivary gland tissue is leading to ecretion of saliva function drop and cause dry mouth.Our previous studies have shown that, Yiqi Yangyin Quyu method Can improve SS in patients with symptoms of dry mouth and improve NOD mouse submandibular gland saliva secretion, damage index. But when the salivary gland immune microenvironment changes，what kind of pathological changes will be in the salivary gland ? How is the process? What is the direct the molecular mechanism on improvement of SS saliva secretion function of Yiqi Yangyin Quyu method? These problems are not exact explanation. Therefore, we proposed to use flow cytometry, confocal microscopy, electron microscopy immunocytochemistry and molecular biology technique. Based on a Comprehensive analysis and observation of the SS gland immune microenvironment, With Yiqi Yangyin Quyu decoction as carrier, observe the effect of its on SS gland immune microenvironment and gland secretion function signal in the whole animal model and vitro cell primary culture trials in order to discuss the mechanism and scientific connotation. To provide a theoretical and experimental basis for Yiqi Yangyin Quyu method in the treatment of SS to improve dry mouth.

干燥综合征(SS)唾液腺组织局部免疫炎症微环境的异常是导致其分泌功能下降造成口干的最主要原因。我们前期研究表明，益气养阴祛瘀法能很好的改善SS患者口干症状和NOD小鼠的唾液分泌量、颌下腺损伤指数。但唾液腺免疫微环境的变化会对其分泌功能产生什么样的病理变化，其过程如何？益气养阴祛瘀法改善SS唾液分泌功能的直接分子机制如何？尚未得以确切阐释。因此，我们拟利用流式细胞术、激光共聚焦显微镜、电子显微镜免疫细胞化学技术和分子生物学等技术，全面系统观察SS患者颌下腺免疫微环境的基础上，以益气养阴祛瘀方为载体，从整体动物模型和离体细胞原代培养多角度探讨其作用机理和科学内涵，为益气养阴祛瘀法治疗SS改善口干提供理论和实验依据

干燥综合征(SS)唾液腺组织局部免疫炎症微环境的异常是导致其分泌功能下降造成口干的最主要原因。前期研究表明，益气养阴祛瘀法能很好的改善SS患者口干症状和NOD小鼠的唾液分泌量、颌下腺损伤指数。但唾液腺免疫微环境的变化会对其分泌功能产生什么样的病理变化，其过程如何？益气养阴祛瘀法改善SS唾液分泌功能的直接分子机制如何？尚未得以确切阐释。本研究拟利用流式细胞术、激光共聚焦显微镜、电子显微镜免疫细胞化学技术和分子生物学等技术，全面系统观察SS颌下腺免疫微环境的基础上，以益气养阴祛瘀方为载体，从整体动物模型和离体组织、细胞培养多角度探讨其作用机理和科学内涵，探究了益气养阴祛瘀方治疗SS影响的具体代谢路径与细胞因子变化，证明了益气养阴祛瘀方能有效改善pSS患者病情活动和颌下腺血流微环境，及益气养阴祛瘀方调节pSS患者颌下腺细胞中水通道蛋白AQP5分布和M3R表达从而控制唾液腺中的水分转运的作用。