绒山羊皮肤毛囊嵌合转录本表达模式研究

Chimeric transcripts arise from the joining of exons from two or more different genes. Reporting such transcripts has become more widespread as the volume of data from genome-wide transcriptional analyses has increased. Recent studies demonstrate that chimeric transcript might play a crucial role in the regulation of gene expression. About 8% reads derived from chimeric transcripts were found in hair follicle transcriptome of cashmere goat. A systematic analysis of the location of the termini of coding genes expressed in various hair follicles will be initiated as part of the pilot project. This project will discover that gene boundaries extend well beyond the annotated termini based on RNA-Seq. Characterization of these chimeric transcripts will highlight that the information stored in the genome and expressed in the transcriptome is not as linear as previously believed. The apparently large discrepancy between the number of putative chimeric transcripts and chimeric proteins reported to date (~100:1) could indicate that most chimeric transcripts are not translated and perhaps serve to regulate processes at the RNA level. To illustrate the proposed characteristic features of chimeras, we focused on the chimeric proteins validated by RNA-seq at the RNA level, as well as those validated by shotgun and targeted mass spectrometry at the protein level. Using multiple analysis approaches we will reveal the biological and evolutionary importance of these chimeric transcripts. The non-random nature of the connection of the genes involved in primary and secondary hair follicle suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network. In this way, we also assess the tissue specificity of the chimeric RNAs and compare the expression of chimeric proteins with that of the parental wild-type proteins. Using high throughput RNA sequencing, mass spectrometry experimental data, and functional annotation, we study specific molecular phenotypes are characterized by expression of chimeric transcripts, lncRNA/miRNA , mRNA and related network.

嵌合转录本由两个或两个以上基因的外显子组成并具有编码新蛋白改变细胞表型的潜力。迄今已通过高通量RNA测序识别了大量可能的嵌合转录本。嵌合转录本以及它们的编码产物通常具有重要的基因调控功能。初步调查发现在绒山羊毛囊转录组数据中有约8%的表达序列来自嵌合转录本。系统分析产生这些转录本的父本编码蛋白基因在山羊基因组上的边界将有助于分析这些嵌合转录本不同于经典转录本的特殊功能。前期研究发现有大约1%的嵌合转录本可以翻译成蛋白质。为深入解析这些嵌合转录本的作用，我们通过RNA水平的转录组分析和蛋白水平的质谱分析确定待研究的候选嵌合转录本。使用多种研究手段将揭示这些嵌合转录本的生物学功能及其在进化中的作用。因为嵌合转录本的非随机特性，我们从整体上以网络的方式研究嵌合转录本的作用。籍此，我们将获得毛囊组织特异性表达的嵌合转录本，并揭示其与特定lncRNA、miRNA和mRNA的相关关系。

嵌合转录本由两个或两个以上基因的外显子组成并具有编码新蛋白改变细胞表型的潜力。迄今已通过高通量RNA测序识别了大量可能的嵌合转录本。嵌合转录本以及它们的编码产物通常具有重要的基因调控功能。初步调查发现在绒山羊毛囊转录组数据中有约8%的表达序列来自 合转录本。系统分析产生这些转录本的父本编码蛋白基因在山羊基因组上的边界将有助于分析这些嵌合转录本不同于经典转录本的特殊功能。前期研究发现有大约1%的嵌合转录本可以翻译成蛋白质。为深入解析这些嵌合转录本的作用，我们通过RNA水平的转录组分析和蛋白水平 的质谱分析确定待研究的候选嵌合转录本。使用多种研究手段将揭示这些嵌合转录本的生物学功能及其在进化中的作用。因为嵌合转录本的非随机特性，我们从整体上以网络的方式研究嵌 合转录本的作用。籍此，我们将获得毛囊组织特异性表达的嵌合转录本，并揭示其与特定lncRNA、miRNA和mRNA的相关关系。