电针傍刺促进皮肤创伤愈合的 CLC 型氯离子通道调控机制研究

Wound healing is important for maintaining the normal structure and function of tissues. During this process, wound current might play a pivotal role. We have found that chloride channels contribute greatly to cutaneous wound current and proved that Bangci electroacupuncture (BEA) can enhance the wound healing of cutaneous wound previously. Recently, we have found out that BEA can decrease chloride ions flux and reduce gene expression of mouse cutaneous wound. So, we assume that BEA might regulate the protein and gene expression of chloride channels during the process of cutaneous wound healing. In this study, we will test the expression levels of mRNA of CLC family members in mouse cutaneous wound by RT-PCR. Then we will confirm protein and gene expression and distribution of selected CLC family members with Western blot and Real-time PCR. Finally, chloride ions flux will be recorded with Non-invasive micro-test. This will help clarifying the regulatory mechnism of chloride channels by BEA for promoting cutaneous wound healing, and enrich the theoretical evidence for cutaneous wound. This study will provide new ways and ideas for cutaneous wound healing.

组织的损伤修复对于维持组织正常结构和功能至关重要，在这一过程中，损伤电流发挥着至关重要的作用。我们研究发现，氯离子流是构成皮肤创伤组织损伤电流的重要组成部分。我们前期研究已证实电针傍刺可以明显促进皮肤创伤组织的愈合；近期预实验又发现，电针傍刺可以明显降低皮肤创伤组织的氯离子流速，抑制部分CLC型氯离子通道基因的表达。故此，我们推测电针傍刺可能是通过调控CLC型氯离子通道蛋白和基因的表达而促进皮肤创伤愈合。本研究以皮肤创伤小鼠为研究对象，采用蛋白印迹、RT-PCR和Real time-PCR等技术检测小鼠皮肤创伤组织CLC氯离子通道蛋白和基因的表达；采用非损伤微测技术检测小鼠皮肤创伤组织氯离子流速；以期阐明电针傍刺对皮肤创伤组织CLC氯离子通道的调控机制，继而丰富了电针傍刺促进皮肤创伤组织愈合的科学内涵。此研究将为皮肤创伤组织的治疗提供新方法和新思路。

组织的损伤修复对于维持组织正常结构和功能至关重要，在这一过程中，损伤电流发挥着至关重要的作用。本研究以皮肤创伤小鼠为研究对象，采用蛋白印迹、RT-PCR 和 Real time-PCR 等技术检测小鼠皮肤创伤组织 CLC 氯离子通道蛋白和基因的表达；采用非损伤微测 技术检测小鼠皮肤创伤组织氯离子流速；以期阐明电针傍刺对皮肤创伤组织 CLC 氯离子通道 的调控机制，继而丰富了电针傍刺促进皮肤创伤组织愈合的科学内涵。本研究研究发现，氯离子流是构成皮肤创伤组织损伤电流的重要组成部分，且电针傍刺可以显著增加氯离子的流速而促进促进皮肤创伤组织的愈合。另外，本研究RT-PCT结果显示9个CLC通道均显著表达，但Western blot 结果显示只有CLC-2, CLC-7, CLC-kα, 和 CLC-kβ表达显著。且电针傍刺可以显著促进CLC-2, CLC-7, CLC-kα, 和 CLC-kβ表达。因此，本研究的结果将为以后的研究提供了新的方法和新思路。