基于TiO2的可见光响应磁控型光电化学免疫传感器用于水产品中河豚毒素的检测方法研究

The photoelectrochemical immunosensor is an analytical method combining specific immunoreaction and sensitive photoelectrochemistry. Nanomaterials with the merits of good biocompatibility, large specific surface area, strong adsorption capacity and easy functionalization, can be used to improve the performance of immunosensors. The magnetic nanoparticles with the advantages of rapid separation under the action of an external magnetic field, can be used to shorten the detection time and simplify the separation. The aim of this project is to construct the visible-light driven magneto-controlled photoelectrochemical immunosenor for detecting tetrodotoxin based on the synthetic or in-situ generated TiO2 nanocomposites coupling with nano signal amplification. Detailedly, the project consisted of the fllowing issues: (1) to establish a novel magneto-controlled photoelectrochemical immunosensor based on GeSe-TiO2 nanocomposites combined with hyaluronic acid coated mesoporous silicon used as signal amplification strategies; (2) to establish a microfluidic photoelectrochemical immunosensor based on melanin-like electron acceptors; (3) to establish a novel visualized photoelectrochemical immunosensing analysis method based on in-situ Bi2S3 used as the sensitizer of TiO2. The research results are expected to provide technical support in the fields of food safety testing and marine environmental analysis.

光电化学免疫传感器是将特异性的免疫反应和高灵敏的光电检测相结合的分析手段。纳米材料具有良好的生物相容性、大的比表面积、强的吸附能力和易于功能化等优点，将其用于免疫传感器中可以提高免疫传感器的分析性能。磁性纳米粒子能在外加磁场作用下实现快速分离，便于富集分离和缩短检测时间。本项目基于制备或原位生成TiO2复合材料并结合纳米信号放大手段构建几种可见光响应磁控型光电化学免疫传感器用于河豚毒素的检测。具体内容：(1)建立以GeSe-TiO2为光电材料结合透明质酸封闭的介孔硅信号放大体系的新型磁控光电化学免疫传感分析方法；(2) 建立基于类黑色素聚合物作为电子受体的新型微流控光电化学免疫传感分析方法；(3) 建立基于原位生成Bi2S3增强TiO2光电化学信号的新型可视化光电化学免疫传感分析方法。本研究成果有望在食品安全检测、海洋环境分析等领域提供技术支持。

免疫传感器是基于抗原-抗体特异性结合反应，具有灵敏度高、选择性强、检测速度快、成本低和操作简单等独特优点的一类生物传感器，有广泛应用前景。而具有强毒性的生物毒素能通过污染食品对人的生命健康造成极大的威胁。本项目将基于纳米材料构建的新型光学和光电化学免疫传感器用于检测生物毒素和肿瘤标记物。具体研究内容包括：（1）以Pt纳米粒子负载氮掺杂的碳纳米材料为过氧化物酶构建一种新型比色/光热双信号免疫传比色感器用于检测黄曲霉毒素B1；（2）以酶控柠檬酸与Fe3+的络合反应构建一种磁控型免疫传感器用于检测赭曲霉毒素（3）以基于酶控反应即时合成的铁氰化铜为模拟酶构建一种磁控型比色免疫传感器用于检测黄曲霉毒素B1；（4）以Ag纳米粒子和压电材料NaNbO3之间的局域表面等离子体共振和压电协同增强效应构建的一种光电化学免疫传感器用于检测甲胎蛋白。本项目的研究工作不但能用于检测生物毒素还可以拓展应用于其它领域的分析检测。