胃癌演进过程中基质金属蛋白酶与乏氧的在体定量可视化方法研究

Accurate diagnosis of early gastric cancer is a key step and effective means to reduce its mortality. The quantitative visualization of some functional molecules in the progression of gastric cancer provides a theoretical basis for the early diagnosis of gastric cancer. In this project, matrix metallo proteinases (MMP-2) and hypoxia were chosen as targets of gastric cancer. Ratiometric NIR fluorescent probes with high selectivity and sensitivity were used to quantitatively visualize MMP-2 and hypoxia in vivo, and MRI imaging with high resolution could dynamically monitor the anatomy of gastric cancer. Taking advantages of multimodality molecular imaging and classical pathological analysis, we could establish an accurate correlation between molecular information, imaging signal, pathological information and tumor progression. Therefore, this project has a significant impact on both fundamental research and practical applications.

胃癌的早期精准诊断是降低其死亡率的关键环节和有效手段，而胃癌关键分子演进的可视化对胃癌的早诊提供了理论基础和科学依据。本项目筛选出与胃癌发生发展密切相关的基质金属蛋白酶（MMP-2）和肿瘤乏氧作为标志物靶标，通过高特异性和高灵敏度的近红外荧光成像实现对关键功能分子MMP-2和肿瘤乏氧的在体定量可视化分析，同时融合高分辨率的磁共振成像以实现对胃癌组织结构变化的动态观测。结合胃癌组织的病理信息，构建分子信息、影像信息和病理信息与肿瘤演进之间的准确关联，为胃癌的筛查和早诊早治提供科学可行的新策略。

胃癌的早期精准诊断是降低其死亡率的关键环节和有效手段，而胃癌关键分子演进的可视化对胃癌的早诊提供了理论基础和科学依据。本项目筛选了与胃癌发生发展密切相关的基质金属蛋白酶(MMP-2)、肿瘤乏氧、微酸、氧化应激作为标志物靶标，构建了一系列高特异、高灵敏的胃癌演进过程中关键分子可视化的探针，实现了对关键功能分子MMP-2、肿瘤乏氧和微酸等标志物的在体定量可视化分析。结合胃癌组织的病理信息，建立了分子信息、影像信息和病理信息与肿瘤演进之间的准确关联，实现早期微小胃癌病灶的高灵敏成像检测、精准区分淋巴转移灶和术中导航。