基于光电化学活性MOFs“多位一体化”生物传感新策略研究

Specific detection of malignant tumor related biomarkers is one of the most effective methods to realize the cancer early diagnosis and early warning. According to the different luminescent mechanisms and structure characteristics of MOFs, the project intends to design and synthesis of a various new metal-organic framework compounds (MOFs) with photoelectric chemical activities. Apply the obtained MOFs to construct electrochemiluminescence and photoelectrochemical sensing platforms for ultrasensitive detection of biomarkers. Investigate the electrochemical, electrochemiluminescence and photoelectrochemical properties of the obtained MOFs modified electrodes. Try to reveal the internal mechanism for the electron transfer and energy conversion during the electric-photo and photoelectric conversion processes. Study the MOFs functionalization methods. Assemble redox mediators, coreactant, carbon dots, and magnetic nanomaterials onto MOFs or into the charnel of MOFs, endowing MOFs with a various functions, to realize “more-in-one” biological sensing detection. Investigate the interaction between the biological molecules and the obtained MOFs, so as to select and design the appropriate biological molecules immobilized method. Construct various immunosensors to detect malignant tumor markers. The implementation of this project will be beneficial to the study of cohesion and cross integration for the related subjects such as chemistry and biology, medicine, material, having a positive significance for the arears of bio-analytical chemistry and clinical medicine.

特异性检测恶性肿瘤相关的生物标志物是实现癌症早期诊断与预警的一个最有效的方法。本项目拟根据金属有机框架化合物(MOFs)不同的发光机制与结构特点，设计、合成多种新型具有光电化学活性的MOFs；将所得MOFs用于构建超灵敏检测生物标志物的电致化学发光及光电化学传感平台。研究光电活性MOFs修饰电极的电化学、电致化学发光及光电化学性质，试图阐明在电光及光电转换过程中的电子转移与能量转化机理；研究MOFs的功能化方式，拟将氧化还原媒介体、共反应试剂、碳点、磁性纳米等组装在MOFs上或孔道间，赋予MOFs多种功能，实现“多位一体”的生物传感检测；研究光电化学活性MOFs与生物分子的相互作用力，从而选择与设计合适的生物分子固载方法；构建多种免疫传感器用于恶性肿瘤标志物的检测。本项目的实施将有利于化学与生物、医学、材料等相关学科研究的衔接和交叉集成，对于生命分析化学与临床医学等领域的发展具有积极意义。

本项目拟根据金属有机框架化合物(MOFs)不同的发光机制与结构特点，设计、合成多种新型具有光电化学活性的MOFs; 将所得MOFs用于构建超灵敏检测生物标志物的电致化学发光及光电化学传感平台。研究光电活性MOFs修饰电极的电化学、电致化学发光及光电化学性质，阐明在电光及光电转换过程中的电子转移与能量转化机理;研究MOFs的功能化方式，将氧化还原媒介体、共反应试剂、碳点、磁性纳米等组装在MOFs上或孔道间，赋予MOFs多种功能，实现“多位一体”的生物传感检测; 研究光电化学活性MOFs与生物分子的相互作用力，从而选择与设计合适的生物分子固载方法; 构建了多种免疫传感器用于恶性肿瘤标志物的检测。