枸杞多糖对兔视网膜色素上皮细胞光损伤的保护作用及其分子机制研究

Age-related macular degeneration (AMD) is a cause of 55 years of age or older is of low vision and the leading cause of blindness, precise etiology and pathogenesis is unknown, and lack of a reliable prevention and treatment method. Research shows that: AMD occurrence and retinal pigment epithelium (RPE) cells light damage closely related , cell apoptosis is the main form of the damage. Traditional Chinese medicine thinks: Fructus Lycii fill the liver and kidney, improving eyesight effect, traditional Chinese medicine is about the eye，lycium barbarum polysaccharide as its main biological active ingredients. We in the early period of the study shows that:Fructus Lycii to RPE cell has certain protective effect, and it relates to oxidation resistance and can improve the damaged RPE cells SOD, raised Bcl-2 protein, cut Bax protein expression about. The research results illustrate the Fructus Lycii to RPE cells light damage protection mechanism of action, in order to further discuss the protection, we will proceed from mitochondria and endoplasmic reticulum mediated cell apoptosis of signaling pathways, by the transmission electron microscope, flow cytometric,Western blot, such as the cell biology and molecular biology experiment technology, the research of lycium barbarum polysaccharide to the rabbit RPE cells light damage the molecular mechanism of protection to prevent AMD, reduce the risk of complication happen and the development of natural medicine treatment of AMD, provide new development ideas and experimental basis.

年龄相关性黄斑变性(AMD)是引起55岁以上老年人低视力和盲的首要原因，确切病因及发病机制未明，因而缺乏可靠的预防和治疗方法。研究表明：AMD的发生与视网膜色素上皮（RPE）细胞光损伤密切相关，细胞凋亡是其主要的损伤形式。中医认为:枸杞具有补肝肾、明目的功效，是中医眼科的常用药，枸杞多糖为其主要的生物活性成分。我们的前期研究显示：枸杞子对RPE细胞具有一定的保护作用，这与其抗氧化性且能提高受损RPE细胞SOD活性、上调Bcl-2蛋白，下调Bax蛋白表达有关。这一研究结果初步阐明了枸杞子对RPE细胞光损伤保护的作用机制，为进一步探讨其保护作用，我们拟从线粒体和内质网介导的细胞凋亡信号传导途径入手，采用透射电镜、流式细胞仪、蛋白免疫印迹法等细胞生物学及分子生物学实验技术，研究枸杞多糖对兔RPE细胞光损伤保护的分子机制，为预防AMD发生、降低罹患风险及开发治疗AMD的天然药物，提供新的研发思路。

年龄相关性黄斑变性(AMD)是引起50岁以上老年人低视力和盲的首要原因，确切病因及发病机制未明，因而缺乏可靠的预防和治疗方法。研究表明：AMD的发生与视网膜色素上皮（RPE）细胞光损伤密切相关，细胞凋亡是其主要的损伤形式。中医认为：枸杞子具有补肝肾、明目的功效，是中医眼科的常用药，枸杞多糖为其主要的生物活性成分。我们的前期研究结果初步阐明了枸杞子对RPE细胞光损伤保护的作用机制，为进一步探讨其保护作用，本项目从线粒体和内质网介导的细胞凋亡信号传导途径入手，采用透射电镜、流式细胞仪、蛋白免疫印迹法等细胞生物学及分子生物学实验技术，研究枸杞多糖对兔RPE细胞光损伤保护的分子机制，研究显示：①光损伤促进了线粒体内膜Cyt-c的释放及细胞内游离Ca2+浓度的升高，导致胞质中Cyt-c及其下游Caspase-9、Caspase-3和Caspase-12浓度的升高；②枸杞多糖通过抑制兔RPE细胞线粒体内膜Cyt-c的释放和细胞内游离Ca2+浓度的升高，进而有效控制了Caspase-9、Caspase-3及Caspase-12蛋白的表达，从而达到保护兔RPE细胞的目的；③枸杞多糖对兔RPE细胞光损伤保护作用可能是通过抑制线粒体和内质网介导的细胞凋亡信号传导通路来实现的。这一结果进一步明确了枸杞子“补肾明目”的作用机制，同时为预防AMD发生、降低罹患风险及开发治疗AMD的天然药物，提供了新的研发思路。