产生免疫保护性的灭活迟缓爱德华氏菌的作用机制

Edwardsiella tarda has become one of the most important emerging pathogens in the current aquaculture, therefore, the design of an effective vaccine to prevent this pathogen is very important. But we find that the inactivated vaccine by the route methods can not produce the immune protection for fish. Based on our pre-invention which can effectively maintain E.tard antigen immune protection in the inactivated vaccine preparation, the new inactivated E.tarda vaccine prepared under different inactivation conditions were investigated using Japanese flounder (Paralichthys olivaceus) as a model. Comparing the results of the antigenic characteristics, the differences in immunogenicity, and its impact of humoral and cellular immune responses of the inactivated vaccine were evaluated. Based on the obtained results we try to explain the reasion to maintain and lose protective immunity for this vaccine. This work will help us to understand the immune protection mechanisms induced by inactivated E.tarda vaccine. This studies also provide a scientific basis for the developed an effective E.tarda inactivated vaccine in future.

迟缓爱德华氏菌（Edwardsiella tarda）是当前水产养殖业中的重要致病菌，对其实用性疫苗的开发具有重要意义，但采用常规技术制备的E. tarda灭活疫苗对鱼体不能产生有效的免疫保护。本项目拟以本实验室前期发明的几种可有效维持E. tarda抗原免疫保护力的灭活方法制备的灭活疫苗为基础，以牙鲆（Paralichthys olivaceus）为实验动物，通过灭活E. tarda的抗原特征、免疫保护性及其对鱼体的体液和细胞免疫激发效应的差异比较，分析灭活条件对E. tarda灭活疫苗得以保持或丢失其免疫保护性的原因，阐释可产生免疫保护性的E. tarda灭活疫苗的作用机制，为E. tarda灭活疫苗的研发提供基础依据。