不同致病型IBV的免疫病理差异与Toll样受体及RIG-I样受体的相关性研究

Avian infectious bronchitis caused by infectious bronchitis virus (IBV), continuous variation in the pathological type. What is the mechanism for variation? What is the reason for pathological types changing since IBV virus were variables tissue tropism? We presumed that variations of IBV pathological type related to immune pathological response in cells. Pattern recognized receptor is a crucial molecular to immune response to entry of pathogen. We studied the different expression of TLRs in variations of pathogen pathological type infection. We hypothesized that different pathogenic type of IBV related to cellular innate immune response. Therefore, we intend to select respiratory types trains and kidney types trains of IBV for the study, infect respiratory and kidney epithelial cells, and detect differences expression of pattern recognition receptor, such as TLR3, TLR7 and Mda5; differences immune response used by methods of CTL killing ability and flow cytometry, then determine the role of TLR3, TLR7 and Mda5 in IBV infection and pathogenicity used by methods of stimulating and inhibition of activity, find the key protein in signal passway. In this study, we intended to find the regularity of variation of pathological IBV, provide targets for drug therapy and research ideas for the common IBV vaccine.

传染性支气管炎病毒（Infectious Bronchitis virus, IBV）致鸡传染性支气管炎，其致病型不断变异。已知IBV为泛细胞嗜性病毒，为何在多组织感染情况下病变差异很大？模式识别受体是识别病原启动免疫应答的第一步，在宿主免疫应答中发挥重要作用。前期研究我们发现禽Toll样受体（TLR）在不同致病型病原感染时不同组织表达有差异。因此推测不同致病型IBV致组织病变差异可能和宿主对IBV的固有免疫应答有关。本研究拟选取呼吸型、肾型IBV为研究对象，分别感染呼吸道和肾上皮细胞，通过CTL杀伤实验和流式细胞术检测呼吸型和肾型毒株感染后的免疫激活情况；通过受体激动、受体功能抑制测定TLR3、TLR7和Mda5在IBV感染和致病中的作用，探索关键蛋白和信号通路。通过本研究可以探索IBV致病型差异的免疫机理，为特效药物治疗提供靶点，为研制IBV共性疫苗提供思路。