蒙医脑-白脉调控针刺对脑卒中后抑郁大鼠额叶脑源性生长因子的影响研究

Post-stroke depression ( PSD ) is one of the common complications of cerebrovascular disease, the study have shown that the incidence of PSD is 25%-80%. Which have negative influence On the recovery of neurological function 、the rehabilitation of Limbs movements and sensory、the quality of life .we have observed in the long-term clinical work that traditional Mongolian medicine brain - white pulse regulating Acupuncture will obviously improve the symptom of depression .the project will built The PSD animal model by middle cerebral artery occlusion and chronic unpredictable mild stress. The rats will be divided into three groups: Acupuncture group、 PSD group and control group. Acupuncture group was a group that PSD rats were Treated by Mongolian medicine brain - white pulse regulating Acupuncture on prefrontal cortex .PSD group was a group that PSD rats will Drench FIuoxetine hydrochloride. control group was a group that PSD rats will not intervene any treatments. he treatment process continued for 3 months. Observing The behavior of animals and expression of brain-derived neurotrophic factor (BDNF) on prefrontal cortex and Investigating Therapeutic effection of Mongolian medicine brain - white pulse regulating Acupuncture on PSD rats and the impaction of BDNF on prefrontal cortex。For providing the Proof of Evidence-Based Medicine to Mongolian medicine Acupuncture theory,widen the disease spectrum of Mongolian medicine Acupuncture theory and looking for more safty、more effective methed of PSD clinical treatment

脑卒中后抑郁（PSD）是脑血管疾病常见的并发症之一，有关研究显示，卒中后抑郁发病率为25%-80%。对患者神经功能恢复及肢体运动、感觉的康复有着消极影响，使生活质量明显下降。我们在长期临床工作中观察到蒙医脑-白脉调控针灸可明显改善抑郁状态，本项目拟应用大脑中动脉栓塞和慢性不可预见温和刺激方法制备大鼠PSD模型。将PSD大鼠分为针刺治疗组(PSD大鼠前额叶行针刺治疗)、药物组(PSD大鼠经胃灌注氟西汀)、空白组（不做任何治疗），干预持续3个月。观察各组大鼠抑郁行为学改变及额叶脑源性生长因子(BDNF)的含量改变，进行对比研究，探讨蒙医针刺对PSD的治疗作用以及对额叶脑源性生长因子的影响。为蒙医针刺治疗PSD提供循证医学依据，拓展蒙医针刺疾病谱，为临床治疗PSD寻求更安全有效的方法。

脑卒中后抑郁（post stroke depression,PSD）是脑卒中后常继发的情绪低落、活动能力减退、思维与认知功能迟缓为临床主要特征的一类情感性精神障碍，是脑卒中后常见的并发症。这些情绪在很大程度上影响了患者神经功能恢复及生存质量。主要表现在住院时间延长、死亡率升高，躯体疾病治疗复杂化，给家庭和社会带来很大的负担。目前治疗除了家庭社会的支持、心里治疗，药物治疗之外，针刺作为一种安全、有效的治疗方法，越来越多地被医者与患者接受。近几年我们用脑-白脉调控针治疗此病取得了较为满意的疗效，克服了西药的不足和局限性,具有方法简单、无副作用，因其作用机制不明而大多数患者依赖于药物，应用受到很大的限制。因此，了解其作用机理是很有必要的。.本项目拟应用了大脑中动脉栓塞和慢性不可预见温和刺激方法制备大鼠PSD模型。把SD大鼠分为针刺治疗组、药物治疗组、假手术组、模型组。观察了各组大鼠抑郁行为学改变及细胞因子、神经递质和前额叶脑源性生长因子(BDNF)的含量改变，进行对比研究，探讨蒙医针刺对PSD的治疗作用以及对额叶脑源性生长因子的影响。为蒙医针刺治疗PSD提供循证医学依据，拓展蒙医针刺疾病谱，为临床治疗PSD寻求更安全有效的方法。