天然纳米颗粒MIR2911靶向TGFβ1用于肿瘤治疗的研究

Malignant tumor is one of the major diseases threatening the health of human beings. In many human cancers, transforming growth factor beta (TGFβ) expression is increased. Further research also indicates that, in the development of tumors, TGFβ promotes the formation and development of tumors through a variety of mechanisms to. Our research group previously found that traditional Chinese medicine honeysuckle encodes a special miRNA, MIR2911, with high expression level and stability. MIR2911 can be absorbed through GI tract and inhibit many influenza virus subtypes replication. Our study found that MIR2911 is more stable than many other miRNAs. With up to 85% of the GC content, we hypothesized that several MIR2911 molecular could combine with each other and form natural nanoparticles, which can easily enter into tumor cells. Bioinformatics analysis also showed that MIR2911 can target TGFβ and inhibit its expression. This project intends to use the natural nanoparticles formed by MIR2911 as a tumor therapeutic drug, by inhibiting the TGF pathway, to achieve the purpose of the treatment of tumor.

恶性肿瘤是威胁人类生命健康的几大主要疾病之一。在人类的多种肿瘤中，转化生长因子β（TGFβ）的表达都增加。进一步的研究也指明，在肿瘤发展晚期，TGFβ可通过多种机制促进肿瘤的形成和发展。本课题组之前的研究发现传统中药金银花中大量稳定表达一种microRNA——MIR2911。MIR2911能被人和动物的消化系统主动吸收，并能够抑制多种流感病毒的复制。我们的研究发现，MIR2911较一般的miRNA更加稳定，结合其高达85%的GC含量，我们推测多个MIR2911能够相互结合并自发形成天然的纳米颗粒，能够更容易进入肿瘤细胞。通过生物信息学手段我们发现MIR2911能够靶向TGFβ，抑制其表达。本项目拟利用MIR2911所形成的天然纳米颗粒作为肿瘤治疗药物，通过抑制TGFβ通路，达到治疗肿瘤的目的。

恶性肿瘤是威胁人类生命健康的几大主要疾病之一。在人类的多种肿瘤中，转化生长因子β（TGFβ）的表达都增加。进一步的研究也指明，在肿瘤发展晚期，TGFβ可通过多种机制促进肿瘤的形成和发展。本课题组之前的研究发现传统中药金银花中大量稳定表达一种microRNA——MIR2911。我们的研究发现MIR2911能够自身相互结合并自发形成天然的纳米颗粒，不需要转染试剂直接被吸收进细胞中。通过生物信息学手段我们发现MIR2911能够靶向TGFβ，抑制其表达。细胞实验证明MIR2911能够在细胞水平上抑制TGFβ的分泌。体内植瘤实验证明，MIR2911能够有效的通过抑制TFGβ通路，抑制肿瘤组织的生长。同时发现酸性环境能够使miRNA形成天然纳米颗粒，直接被细胞所吸收。MIR2911所形成的天然纳米颗粒可作为肿瘤治疗的潜在药物，造福社会。