第三代生物传感器的构件及原理研究

In the present thesis, the third-generation biosensor is prepared by making use of the unique effects of nanoparticle materials, especial the metal nanocrystal. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilization enzyme. The current response of the biosensor is increased significantly. Based on our experiment, it could be accounted for the combined effect of the conductivity, macroscopic quantum tunneling, chemical activity, catalytic activity and microarray electrode effect, in addition to surface effect of metal nanoparticles. The results will make a basis of improving the performance of biosensor, and developing the direct electron transfer biosensor, open up a new road to miniaturize the biosensor. It can make a basis of research, preparation and use of the biosensor enhanced by nanoparticles.

本项目应用超分子自组装成膜技术，经软化学途径，实现酶蛋白分子和纳米颗粒的定向组装和纳米层次有序结构的调控，研究酶/纳米颗粒薄膜的有序化组装规律和机理，构建酶与电淠苤苯拥缱哟莸牡谌干锎衅鳌Ｌ岣呙傅鞍追肿邮侗鸨∧さ男畔⒋碜患复菽芰Γ诘谌咸烟巧锎衅鞯脑硌芯炕∩希兄埔谎趸厦肝⑿蜕锎衅鳌