氧化石墨烯杀菌作用及其机制研究

Graphene oxide (GO),due to its potential applications such as drug delivery, cell imaging, environmental protection and biosensor manufacture, has attracted lots of attentions. The low cytotoxicity of GO makes it a potential antibacterial material. However, the antibacterial effect of GO and its corresponding mechanisms are still unclear. Model organism of Caenorhabditis elegans (C. elegans) has been widely applied in many research fields, such as screening of drugs or target proteins, study of toxicology and antibacterial substances, the survival situation of C. elegans and bacterial activity in vivo could reflect the antibacterial effect of materials. In this project, we will analyze the antibacterial effect of GO and PEG-GO against Pseudomonas aeruginosa and their underlying, and further study the antibacterial effect of a compound, which is composed of PEG-GO and piperacillin, against Pseudomonas aeruginosa and its underlying. This research will elucidate the antibacterial effect and mechanisms of GO and PEG-GO against Pseudomonas aeruginosa, and provide basis for the application of PEG-GO as potential drug delivery carrier.

氧化石墨烯（GO）是在药物载体、细胞成像、环境保护、生物传感器制造等领域具有潜在应用价值的一种纳米材料，并且具有很小的细胞毒性，有望成为一种极好的抗菌材料，但针对氧化石墨烯杀菌作用及可能的作用机理尚不完全清楚。模式动物秀丽线虫已广泛应用于药物或靶蛋白筛选、病毒作用机制研究，毒理学研究以及杀菌物质研究等领域，线虫的存活情况及体内细菌活性的变化可有效反映物质的杀菌作用。为此本项目将分析GO和PEG-GO对于绿脓杆菌的杀菌作用及其作用机制（包括体外杀菌作用和体内杀菌作用），并且将临床常用的抗绿脓杆菌感染的药物（哌拉西林）负载到PEG-GO上形成复合物，研究其对于绿脓杆菌的杀菌作用及其作用机制。该项目将可以系统解析GO和PEG-GO对于绿脓杆菌的杀菌作用，解析其内在分子机制，并且为PEG-GO作为药物靶向输送载体的应用提供相应的理论和技术基础。