马尾松高产脂性状候选基因挖掘与关联分析研究

Resin was an important forest productand ，and it was also an important material in pharmaceutical, chemical and perfume industry. More than 90% of the rosin was collected from Masson pine in China. Resin had become a major source of income pine plantation operations due to the market in short supply. Forestry production and seedlings market demand for high-yield resin varieties continued to increase. The improvement of Masson pine resin productivity was started late. Currently, the varieties of high-yielding resin still less. In order to meet the needs of forestry production, there need to speed up the breeding of high-yielding resin on Masson pine. Molecular marker-assisted selection was a feasible method ..In this study, it will be used sequencing and bioinformatics analysis on Masson pine genome, and finding candidate genes with the high-yielding resin traits. Based on these candidate genes, it will development SSR and SNP markers. By these markers, it dose association analysis with High-yield Resin on Masson pine. To select the appropriate molecular markers carry marker-assisted selection on high-yielding resin on Masson pine.

松脂是我国的重要林产品之一，是医药、化工、香料产业的重要原材料。我国有90%以上的松脂是采自马尾松。随着松原脂市场价格不断走高，采割松脂已成为松树人工林经营的主要收入来源。林业生产及种苗市场对马尾松高产脂良种的需求不断提高。而我国马尾松的产脂改良研究起步相对较晚。目前能够推广的马尾松高产脂良种数量仍然较少。为了尽快选育大量的马尾松高产脂良种，以满足林业生产的需求。开发研究与高产脂性状具有较强相关性的遗传标记，进行分子标记辅助选择，是加快马尾松产脂性状改良进程的一种可行方法。.本研究拟通过对马尾松基因组进行测序及生物信息学分析，挖掘与产脂性状相关的候选基因。基于此开发SSR和SNP标记，进行产脂性状的关联分析。以选择适合的分子标记开展马尾松高产脂分子标记辅助选择研究，加快遗传改良进程。

松脂是我国的重要林产品之一，是医药、化工、香料产业的重要原材料。随着松原脂市场价格不断走高，采割松脂已成为松树人工林经营的主要收入来源。林业生产及种苗市场对马尾松高产脂良种的需求不断提高。为了尽快选育大量的马尾松高产脂良种，以满足林业生产的需求。本研究基于简化基因组关联分析和候选基因关联分析对马尾松产脂量性状开展分子标记辅助选择研究，并挖掘与产脂性状相关的重要基因。项目共获得了与产脂量性状显著相关的SNPs位点3个，平均表型变异解释率12.19%；SSR标记9个，平均表型变异解释率0.92%。验证3个SNP标记在辅助选择研究上的实用性，发现Marker249970位点的AT基因型在2个验证群体中都表现高产性，高产单株比例达到83.33%。已可以基于该标记进行早期的高产脂杂交育种亲本筛选工作。证实了在马尾松产脂性状改良研究中运用分子标记辅助选择的可行性，为马尾松及其它他林木开展分子标记辅助育种研究提供了具有积极意义参考经验。项目还得到了DXS、FPPS、GGPPS等与马尾松产脂性状相关的基因，深入解析马尾松产脂机理提供基础。