假俭草持绿性状-候选基因关联分析及持绿相关关键基因、紧密关联分子标记挖掘

Centipedegrass (Eremochloa ophiuroides) also known as "Chinese lawn grass", is native to southern China. Centipedegrass is a warm-season turfgrass species with less water and fertilizer demand, low maintenance, and strong resistance. It is important to the green development of turf industry that excavating resources of Chinese Centipedegrass and developing new Centipedegrass varieties. The green period affects the performance of turf grass and has been widely studied, but the stay-green as an intrinsic factor in determining the green period is lack of attention. It is difficult to cultivate new varieties with good stay-green by traditional breeding means, for stay-green is regulated by multiple genes. Therefore, it is urgent to explore the molecular mechanism of the stay-green of Centipedegrass, and to explore the key genes and associated molecular markers. In this study, the stay-green property of Chinese will be evaluated, related stay-green gene and SSRs、SNPs and InDels will be explored through 2+3 generation transcriptome sequencing and candidate gene association analysis. Related key genes and significant associated molecular markers will be explored, the molecular mechanism of the stay-green will be revealed, and molecular breeding of Centipedegrass will be promoted through transcriptomics, miRNAs, and candidate gene association analysis.

假俭草（Eremochloa ophiuroides）起源于我国南方，又称“中国草坪草”，具水肥需求少、耐粗放管理、抗逆强等特点，是一种优良的暖季型草坪草。充分挖掘我国假俭草资源、培育新品种，对草坪业节能绿色发展意义重大。绿色期影响草坪草的坪用性能，得到广泛研究，但持绿性作为决定绿色期的内在因素，缺乏关注。持绿性受多基因调控，利用传统育种手段进行假俭草持绿新品种培育难度大。因此急需探究假俭草持绿分子机制，挖掘关键基因及关联分子标记。本项目系统评价我国假俭草的持绿性，利用自主培育“武陵假俭草”品种为材料，进行2+3代转录组及miRNA测序，挖掘持绿相关基因及开发SSR、SNP和InDel等分子标记，并进行候选基因关联分析。最后综合利用转录组、miRNA组和候选基因关联分析的信息，揭示假俭草持绿分子机制，挖掘持绿相关关键基因及紧密关联分子标记，促进假俭草分子育种。

假俭草（Eremochloa ophiuroides）起源于我国南方，又称“中国草坪草”，具水肥需求少、耐粗放管理、抗逆强等特点，是一种优良的暖季型草坪草。充分挖掘我国假俭草资源、培育新品种，对草坪业节能绿色发展意义重大。绿期影响草坪草的坪用性能，得到广泛研究，但持绿性作为决定绿期的内在因素，缺乏关注。持绿性受多基因调控，利用传统育种手段进行假俭草持绿新品种培育难度大。因此急需探究假俭草持绿分子机制，挖掘关键基因及关联分子标记。本项目研究了外源激素、离子钛对假俭草抗寒、种子生产、坪用性能等指标的影响，系统评价我国假俭草的持绿性，利用自主培育“武陵假俭草”品种为材料，进行2+3代转录组，挖掘持绿相关基因及开发SSR和SNP分子标记，进行了假俭草内参基因的挖掘和候选基因关联分析，正在进行持绿性状相关基因验证。最后综合利用转录组、miRNA组和候选基因关联分析的信息，揭示假俭草持绿分子机制，挖掘持绿相关关键基因及紧密关联分子标记，促进假俭草分子育种。通过项目实施挖掘了紧密相关SSR分子标记3个，分别为SSR9、SSR14和SSR29；构建了2 代转录组信息库、3 代转录组信息库和mRNA信息库；挖掘了持绿相关的基因5个，分别为bZIP39、PIF4、EIL3、CCA1和PRR7，正在进行拟南芥体外表达验证；开发了假俭草低温胁迫下表达稳定性的内参基因UBC和MD；揭示了影响假俭草持绿性状的主要因子为低温胁迫，超氧化物歧化酶、过氧化氢酶等氧化还原指标是影响假俭草持绿性状的主要生理指标，精氨酸脯氨酸代谢、植物昼夜节律、植物激素信号转导和类黄酮生物合成等生物通路是影响假俭草持绿性状的主要分子通路。