短短芽孢杆菌驱动水体中共存的溴代阻燃剂和有机磷阻燃剂微生物降解的复合效应及机制

Brominated flame retardants (BFRs) and organophosphate flame retardants (OPFRs) are frequently detected in contaminated water, and the ecological risks for human health have attracted widespread attention. Thus, it has been a research hotspot to develop effective remediation technology. In this project, typical BFRs (BDE-209 and BDE-47) and OPFRs (TPhP and TCP) detected in e-waste contaminated Brominated flame retardants (BFRs) and organophosphate flame retardants (OPFRs) are frequently detected in e-waste contaminated water, and the ecological risks for human health have attracted widespread attention. Thus, it has been a research hotspot to develop effective remediation technology. In this project, typical BFRs (BDE-209 and BDE-47) and OPFRs (TPhP and TCP) detected in e-waste contaminated water are taken as target pollutants. This researh aims to study adsorption and degradation characteristics of single and co-existed BFRs and OPFRs, to investegated bacterial cell characteristics, microbial activity, and microbial community structure changes during BFRs and OPFRs microbial remediation, to analyzed the functional gene and protein through proteomics and transcriptome study, to reveal the mechanisms of BFRs and OPFRs microbial adsorption, membrane transportation, and transformation from the aspect of pollutants transformation, cellular reposne, gene and protein expression. The outcomes of this research will provide basis and techinical support for microbial remediation of BFRs and OPFRs co-existed in water.

水体中的溴代阻燃剂（BFRs）和有机磷阻燃剂（OPFRs）污染及其生态风险问题已经引起了广泛的关注，开发有效的降解修复技术是一项研究热点。本项目拟以污染水体中典型的BFRs（BDE-209和BDE-47）和OPFRs（TPhP和TCP）为目标污染物，研究了BFRs和OPFRs单一污染和复合污染体系下的微生物吸附和降解特性；分析了BFRs和OPFRs复合污染物在降解过程过程中菌体细胞特性变化、微生物活性和微生物群落结构的变化；通过蛋白组学和转录组学探索BFRs和OPFRs降解的功能基因和蛋白，从物质的转化、细胞响应、基因和蛋白表达多个角度揭示微生物修复水体BFRs和OPFRs复合污染过程中污染物吸附、跨膜转运、代谢转化的机制，为微生物强化降解在BFRs和OPFRs复合污染水体治理中的实际应用提供理论依据及技术支持。

电子垃圾污染水体中的溴代阻燃剂（BFRs）和有机磷阻燃剂（OPFRs）污染及其生态风险问题已经引起了广泛的关注，开发有效的降解修复技术是一项研究热点。本项目拟以污染水体中典型的BFRs（BDE-209和BDE-47）和OPFRs（TPhP和TCP）为目标污染物，以一株从电子垃圾污染水体沉积物中筛选分离并证实同时对BFRs和OPFRs具有降解作用的细菌—短短芽孢杆菌（Brevibacillus brevis）为实验菌株，研究了BFRs和OPFRs单一污染和复合污染体系下的微生物吸附和降解特性；分析了BFRs和OPFRs复合污染物在降解过程过程中菌体细胞特性变化、微生物活性和微生物群落结构的变化；通过蛋白组学和转录组学探索BFRs和OPFRs降解的功能基因和蛋白，从物质的转化、细胞响应、基因和蛋白表达多个角度揭示微生物修复水体BFRs和OPFRs复合污染过程中污染物吸附、跨膜转运、代谢转化的机制，为微生物强化修复在BFRs和OPFRs复合污染水体中的实际应用提供理论依据及技术支持。