人工湿地植物根系泌氧对温室气体生成影响机理研究

Constructed wetland was widely used to treat waste water in China. But it was attracting more and more interests that CH4 and N2O as the greenhouse gases emitted at the same time. In this study, the influence mechanism of radial oxygen loss from roots on greenhouse gas production from integrated vertical-flow constructed wetland was discussed. It researched on the bacterial population diversity and com-munity structure based on the analysis of 16S rDNA sequences of system in optimum processing conditions, which took the method of molecular biology, and constructed the 16S rDNA gene library. The concentrations of CH4 and N2O were analysed by GC. According to the research results, the emission characteristics of greenhouse gases at different growing stages of wetland plants were revealed. Also, the influence mechanism of radial oxygen loss from roots on greenhouse gas production was analysed. The research results were helpful to choose wetland plants and reduce greenhouse gases emission.

随着人工湿地污水处理技术在我国的大面积推广，其在消减环境污染物的同时释放大量的温室气体甲烷和一氧化二氮，这一问题越来越引起人们的广泛关注。本课题针对这一问题，拟针对广泛应用的复合垂直流人工湿地技术，开展湿地植物根系泌氧对温室气体生成的影响机制研究。运用环境微生物学技术，借助气相色谱分析手段定量测定温室气体时空排放浓度，通过构建DNA文库、PCR-DGGE等生物学方法表征微生物多样性及种群特征，阐明根系泌氧作用对根系微环境的影响过程，揭示湿地植物不同生长时期温室气体生成及释放规律，明确泌氧作用对温室气体生成的影响机理。研究成果对于湿地植物选择、保障人工湿地净化效果及温室气体减排具有重要的意义。

随着人工湿地污水处理技术在我国的大面积推广，其在消减环境污染物的同时释放大量的温室气体甲烷和一氧化二氮，这一问题越来越引起人们的广泛关注。该研究采用广泛应用的复合垂直流人工湿地技术，研究了人工湿地污染物去除效率与温室气体排放时空变化特征，探明了污染物去除效果与温室气体排放直接的内在联系；表征了湿地植物根系表面微生物多样性及种群变化特征，揭示了湿地植物根系泌氧对温室气体生成的影响机制。研究成果对于湿地植物选择、保障人工湿地净化效果及温室气体减排具有重要的意义。