核糖体蛋白RPS15A通过调控EGFR在食管鳞癌进展中的作用及机制研究

Ribosomal proteines are usually expressed abnormally in cancer cells and affect cancer progression, which is a hot spot in current cancer targeted therapy research. The protein encoded by the RPS15A is a constituent of the ribosomal 40s subunit. More and more studies have confirmed that RPS15A is involved in the process of tumorigenesis and development, At present, it has become a hot spot in tumor-targeted therapy research. However, the role of RPS15A in esophageal squamous cell carcinoma (ESCC) has not been reported. In the phase of my doctor study, we found it that: in clinical specimens, the expression of RPS15A in ESCC is much higher than that in paired para-carcinoma tissue. Similarly, RPS15A is highly expressed in human ESCC cell lines, the proliferation of ESCC cells was significantly inhibited and the apoptosis was significantly increased. Conjoint analysis of Transcriptomics and Proteomics data showed that RPS15A through the EGFR signaling pathway involved in the progression of esophageal squamous cell carcinoma, but the specific mechanism is not clear. Based on these preliminary data, specific contribution of RPS15A to the progression of ESCC which detecting by molecular and cell biology experimental technologies. Then, we will make further research on the molecular process of EGFR pathway regulated by RPS15A in ESCC cells. The novelty and significance of current study lies on several aspects: our study will reveal the function and molecular mechanism of RPS15A-EGFR signaling axis in ESCC. Moreover, our study might identify the potential targets and yielding novel therapeutic approaches for ESCC.

越来越多的研究证实RPS15A参与癌症进展，但机制不详，且RPS15A在食管鳞癌中的相关作用尚未有报道。我们前期研究发现：食管鳞癌组织RPS15A表达水平远高于癌旁组织；RPS15A缺失可抑制食管鳞癌细胞生长，促进细胞凋亡；RPS15A调控食管鳞癌进展与EGFR信号通路密切相关，但具体机制尚不清楚。基于此，本项目拟以生物信息学为切入点，采用分子生物学和细胞生物学手段：研究RPS15A参与食管鳞癌进展的具体贡献；阐明RPS15A调控食管鳞癌进展可能的具体机制-RPS15A调控EGFR下游信号转导的分子机制；从转录及翻译水平分析RPS15A介导EGFR表达的关键调控机制。本项目旨在阐明RPS15A调控EGFR表达影响食管鳞癌进展的确切机制，项目的实施不仅有助于全面认识RPS15A在食管鳞癌进展中的具体作用，也可能为发现新的食管鳞癌诊疗靶点提供线索。

越来越多研究证实RPS15A参与癌症的进展，但RPS15A在食管鳞癌中的相关作用尚未有报道。我们的研究发现：食管鳞癌组织RPS15A表达水平远高于癌旁组织；RPS15A缺失可抑制食管鳞癌细胞生长，促进细胞凋亡；RPS15A调控食管鳞癌进展与EGFR信号通路密切相关。本项目以生物信息学为切入点，采用分子生物学和细胞生物学手段：研究了RPS15A参与食管鳞癌进展的具体贡献；阐明了RPS15A调控食管鳞癌进展可能的具体机制-RPS15A调控EGFR下游信号转导的分子机制；从转录及翻译水平分析了RPS15A介导EGFR表达的关键调控机制。本项目阐明RPS15A调控EGFR表达影响食管鳞癌进展的确切机制，不仅有助于全面认识RPS15A在食管鳞癌进展中的具体作用，也可能为发现新的食管鳞癌诊疗靶点提供线索。我们的发现可能为阐明RPS15A在ESCC发生发展、复发、转移抵抗中的潜在作用提供重要的启示。