坛紫菜孢子囊枝形成过程高磷需求引发的脂类重构及激素调控的研究

Pyropia haitanensis has an important economic and ecological value. At present, the seedling of Pyropia haitanensis was basically in the form of conchocelis filaments in the room under artificial control, by cultivating conchocelis filaments for conchospores. A large number of the formation of the sporangial branchlets was the key to the Pyropia haitanensis seedling. If the size of the sporangial branchlets could be achieved, the rate of mature of the conchocelis filaments could be improved, and the optimal production efficiency of the conchospores could be achieved. Therefore, the research on the regulation of the development of the sporangial branchlets was the key point of the biology and culture of Pyropia haitanensis. In the stage of filaments to sporangial branchlets, it is widely used to supplement the high content of phosphate to promote the formation of sporangial branchlets. However, the internal mechanism was few studied and reported. The cultivated conchocelis filaments of Pyropia haitanensis was used in this study. Lipidomics technology and RNA-Seq (Quantification) were used to study the effect of high concentrations of phosphorus to lipid reconstruction, and explore the regulation of hormone to the development of sporangial branchlets during the process of sporangial branchlets. This study will provide scientific knowledge and guidance for the production of Pyropia haitanensis, achieve the objective of the seedling scientific regulation, and promote the sustainable development of aquaculture.

坛紫菜由于具有重要的经济价值和生态价值而被研究。目前，坛紫菜育苗基本都以丝状体的形式在室内人工控制条件下进行，通过培养丝状体来获得壳孢子。培养过程中孢子囊枝的大量形成是坛紫菜育苗中的重要环节，如能实现孢子囊枝细胞的规模化形成，就能提高丝状体的成熟率，以达到最佳的采集壳孢子的生产效果。因此开展坛紫菜孢子囊枝发育调控的研究是坛紫菜生物学和栽培学的重点。在坛紫菜丝状体发育形成孢子囊枝的阶段，生产中普遍采用补充高含量磷酸盐的方法来促进孢子囊枝形成，但其内在机理鲜有研究和报道。本研究拟以室内人工培养的坛紫菜丝状体为研究对象，主要采用脂质组学与转录组学技术，研究坛紫菜孢子囊枝形成过程高磷需求引发的脂类重构机制，同时探讨激素调控坛紫菜孢子囊枝的形成。本研究可为坛紫菜生产性育苗提供科学知识和指导，达到坛紫菜育苗科学调控的目的，进而促进坛紫菜养殖业的可持续发展。

坛紫菜具有重要的经济价值和生态价值，坛紫菜培养过程中孢子囊枝的大量形成是坛紫菜育苗中的重要环节。本项目以坛紫菜孢子体为材料，研究了不同磷浓度对坛紫菜孢子体发育的影响，筛选出了促进坛紫菜孢子囊枝形成的最佳磷浓度为N/P=1:10；研究了坛紫菜孢子囊枝形成过程脂类的变化规律，发现总的糖脂和磷脂在坛紫菜孢子囊枝形成过程的初期增加，然后再减少后又增加；研究了坛紫菜孢子囊枝形成过程激素的变化规律，发现激素含量（除苯乙酸）在坛紫菜孢子囊枝形成过程的初期较高，之后伴随孢子囊枝成熟率增加而减少；利用转录组分析了坛紫菜孢子囊枝形成过程的表达差异，筛选出了坛紫菜孢子囊枝形成过程的关键差异基因有1106条；同时研究了几种外源激素对坛紫菜孢子体生长的影响，筛选出了促进坛紫菜孢子体生长的最佳外源激素为苯乙酸和茉莉酸。本研究阐明了坛紫菜孢子囊枝发育过程中脂类重构和激素的变化规律，为今后坛紫菜生产性育苗具有重要指导意义。. 标注项目资助已发表SCI论文4篇。