坛紫菜分子克隆和测序

Three distinctive AFLP sequences of Porphyra haitanenesis were cloned and sequenced. According to the sequences of two among three, the routine primers of PCR which could be used in the identification of germ plasm were designed .One patent of pure line development was proclaimed and five pure lines of Porphyra haitanenesis have been developed by using the means. Four among the five pure lines with excellently economic character have a good prospect of cultivation.

栽培坛紫菜分化出一具生长优势的新类型，其特异性的AFLP分子标记已经找到。把这些片段进行克隆和测序，根据序列住处设计出常规的PCR引物，并用它们对生长慢的栽培类型和自恢纸欣┰觯页鐾葱蛄小Ｕ舛杂谟胫匾眯宰从泄鼗虻淖钪杖范ā⒈４婧推鹪囱芯浚糯嘌员；ず桶颜庖辉耘嗬嘈团嘤捎帕嫉脑耘嗥分侄加泻苤匾囊庖濉