空心莲子草表型可塑性和环境适应性的分子机制及其入侵性分析

Alternanthera philoxeroides has strong phenotypic plasticity and environmental adaption characters, which invade in 20 provinces and cities of China. It is serious threat to farming region and the ecological safety and has been included in the "The first list of alien invasive species in China." by the ministry of environmental protection to prevent and control the spread of Alternanthera philoxeroides. The prevention and control the spread of Alternanthera philoxeroides has become an important task of the implementation of the national ecological security strategy. The research aimed is to explore the relationship between the phenotypic plasticity and environmental adaption mechanism of Alternanthera philoxeroides and its invasive internal relations, which will lay a theoretical foundation for development of scientific and efficient and sustainable prevention and control technology. This project intends to combine high-throughput methods (including metabolic, transcriptome and microRNA-sequencing), molecular biology methods and cytology means for finding the important phenotypic plasticity and environmental adaption genes of Alternanthera philoxeroides and their regulatory networks, in order to elucidate the molecular mechanism of phenotypic plasticity and environmental adaption in Alternanthera philoxeroides. Investigation of the ecological characteristics of Alternanthera philoxeroides will dissect the inner relationship between Alternanthera philoxeroides phenotypic plasticity and environmental adaption and invasibility. It will provide a strong theoretical basis for the exploration of Alternanthera philoxeroides invasion ecology mechanism and setting the new model of ecological prevention and control measures.

空心莲子草具有极强的表型可塑性和环境适应能力，广泛入侵我国20多个省市，严重破坏当地的生态平衡，被环境保护部列入“中国第一批外来入侵物种名单”中。预防与控制空心莲子草的蔓延危害已成为实施国家生态安全战略的重要任务之一。研究解析空心莲子草的表型可塑性和环境适应性与其入侵性内在关联关系，将为开发科学高效和可持续的防控技术奠定理论基础。本项目拟采用高通量测序手段（转录组、microRNA 以及代谢组），研究空心莲子草在三种生境下的差异表达基因和代谢产物之间的关系，筛选与空心莲子草表型可塑性和环境适应性相关的重要基因，剖析各代谢途径和调控网络与空心莲子草高表型可塑性和环境适应性的内在联系，阐明空心莲子草具有较高表型可塑性和环境适应性的分子机理。利用空心莲子草表型变化生理特征，进一步解析空心莲子草表型可塑性和环境适应性与入侵性的内在关联，为开发新型生态防控措施提供有力的理论依据。

空心莲子草具有极强的表型可塑性和环境适应能力，广泛入侵我国20多个省市，严重破坏当地的生态平衡，被环境保护部列入“中国第一批外来入侵物种名单”中。预防与控制空心莲子草的蔓延危害已成为实施国家生态安全战略的重要任务之一。研究解析空心莲子草的表型可塑性和环境适应性与其入侵性内在关联关系，将为开发科学高效和可持续的防控技术奠定理论基础。本项目采用高通量的转录组测序、microRNA测序以及代谢组学对三种不同生境下的空心莲子草差异表达基因和代谢产物分析，经GO功能富集分析和KEGG代谢途径分析对其表型可塑性和适应性基因进行挖掘和鉴定，系统研究不同生境下空心莲子草的转录组变化以及转录后调控变化，筛选与空心莲子草表型可塑性和环境适应性相关的重要基因，构建相关的调控网络，进而初步揭示其可塑性和环境适应性的分子机理。三种组学不同产物差异联合分析，确定空心莲子草表型可塑性和环境适应性相关的重要途径和基因主要富集在植物激素、次生代谢产物和光合作用等途径。并通过DNA末端快速扩增技术（RACE）克隆候选基因的全长cDNA，构建植物VIGS表达载体，通过农杆菌介导，获得转基因阳性植株，对其功能进行验证，确定空心莲子草表型可塑性和环境适应性相关基因，进一步解析空心莲子草表型可塑性和环境适应性与入侵性的内在关联，为探索空心莲子草入侵的生态学机制和开发新型生态防控措施提供有力的理论依据。