硫化氢调节红树植物生长发育的生理与分子机制

H2S is recognized to regulate growth, development and various physiological processes in organisms. Mangrove wetland is featured by high H2S release due to its unique reductive habitat with high sulfate substrates. However, whether H2S affect mangrove plant growth and development, particularly the regulation mechanisms at physiological and molecular level, are largely not clear yet. In this project, by using the techniques of gas exchange, membrane two-phase separation, HPLC, inductively coupled plasma mass spectrometry, X-ray microanalysis, non-invasive ion selective electrode, proteomics, transcriptomics, qRT-PCR, Western blot and as well as using typical enzymological methods, growth analysis and NaHS, a donor of H2S, we will investigate the following items. 1) To study the regulation of H2S on growth, root development, leaf photosynthetic characteristics, stomatal movements of mangrove plant Avicennia marina under NaCl treatment. 2) To study the effects of H2S on leaf salt secretion, Na+ and sulfate flux across root tips. 3) To understand the effects of H2S on ascorbate-glutathione homeostasis and antioxidation activity. 4) To study the production of endogenous H2S and the activities and gene expressions of L-cysteine desulphydrase (LCD) and O-acetylserine(thiol)lyase (OASTL), which are the key enzymes in S metabolism. 5) To analyze the differentially expressed protein profiling and gene expression changes at transcriptional level induced by H2S. The proposed results will improve the knowledge of the functions of H2S in growth, development and physiology of mangrove plant, and further to understand the adaptation of mangrove plant to unique reductive environment at the physiological and molecular level.

H2S已被证明能调节生物生长发育和生理过程。红树林湿地由于高硫酸盐和还原性生境从而具有较高H2S水平。但H2S是否影响红树植物生长发育仍不清楚，特别是H2S在生理和分子水平上的调节机制需进一步研究。本项目运用气体交换、膜两相分离、液相色谱、ICP、X 射线微区分析、离子非损伤测定、蛋白组学和转录组学技术、qRT-PCR和Western blot等手段，用H2S 供体NaHS 模拟H2S 释放，研究：(1) H2S对盐处理下红树植物白骨壤生长、根系发育、叶片光合特征和气孔运动的调节；(2)H2S 对叶片泌盐、根部钠和硫酸盐离子吸收的影响；(3) H2S 对谷胱甘肽-抗坏血酸稳态及抗氧化能力的影响；(4) H2S 对硫代谢过程关键酶LCD 和OASTL 活性及基因表达和内源H2S产生的调节；(5) 分析H2S诱导的差异表达蛋白和基因。结果将在生理和分子水平上提高人们对红树植物环境适应性的认识。

红树林湿地由于高硫酸盐和还原性生境从而具有高H2S水平。但H2S是否影响红树植物生长发育仍不清楚，特别是H2S在生理和分子水平上的调节机制需进一步研究。本项目运用气体交换、ICP-MS、X 射线微区分析、离子非损伤测定（NMT）、蛋白组学和转录组学技术、qRT-PCR和Western blot等手段，用H2S 供体NaHS和其他供体 模拟H2S 释放，研究了：(1) H2S对盐处理下红树植物白骨壤生长、根系发育、叶片光合特征和气孔运动的调节；(2)H2S 对叶片泌盐、根部钠和钾离子吸收的影响；(3) H2S 对谷胱甘肽-抗坏血酸稳态及抗氧化能力的影响；(4)H2S 对硫代谢过程关键酶LCD 和OASTL 活性及基因表达和内源H2S产生的影响；(5)H2S对蛋白和基因表达谱的调节。结果在生理和分子水平上提高了人们对红树植物环境适应性的认识。项目共发表了标注有资助号的学术文章17篇，其中SCI论文16篇（JCR一区5篇、5篇、三区3篇、四区3篇），中文核心期刊文章1篇；培养3名硕士毕业论文，3名博士毕业论文。