肠易激综合征大肠粘膜新基因的筛选

The irritable bowel syndrome(IBS) is one of most common digestive diseases,which is termed as one of functional bowel disorders.. The pathophysiology of IBS has not been unclear and IBS gene expression has not been reported so far. The gene expressions in colon mucosa were.investgated through gene microarray in diarrhea-IBS and constipation- IBS. The gene expressions were increased for IL-1β,VIP in diarrhea-IBS group, and decreased for somatostatin,leukotriene(LT) and receptor of 5-HT.in constipation- IBS group. In this study, the model of.Wistar was also established for the constipation- IBS. In this model, LTB4、、5-HT in the plasma was signifcant increased in constipation-Wistar group, and mast cell and 5-HT in mucosa nerve plexus were also increased as well. The above results indicated that pathophysiology of IBS was related to abnormal gene expression in colon mucosa and immuno-nerve cells in colon

据国内外研究和我们的工作，提出筛选肠易激综合征新基因的依据、思想和新方法。设计了本病大肠粘膜相关基因的筛选范围，基因数量1842个。通过应用基因微矩阵及后转录PCR法筛选腹泻及便秘型肠易激综合征异常表达新基因，探讨二者的分子机制及其不同。对诖踊蛩窖芯勘静〔±砘啤⑻教制湔锒虾头乐蔚男峦揪都捌浜蠡蜃榈难芯烤哂兄匾庖濉