分子印迹电化学发光能量转移方法的建立及分析应用

Molecularly imprinted electrochemiluminescence resonance energy transfer, the combination of electrochemiluminescence resonance energy transfer and molecularly imprinted technology, is promising in biochemical analysis owing to its advantages of high sensitivity and selectivity, low cost and good controllability. In this project, we are planning to synthesize low toxic, high efficient and good stable electrochemiluminescent molecularly imprinted polymers via sol-gel and electropolymerization methods, establish electrochemiluminescence resonance energy transfer motif, develop sensitive and specific molecularly imprinted electrochemiluminescence resonance energy transfer method, investigate its analytical applications in explosives and antitumor drugs and discuss the mechanisms of sensing platform for electrochemiluminescence resonance energy transfer. It lays a sound foundation for exploring electrochemiluminescent systems and detecting small molecules in complex samples.

分子印迹电化学发光能量转移技术，是电化学发光能量转移和分子印迹技术的结合，具有灵敏度高、特异性好、成本低、可控性好等优势，在生化分析检测领域应用前景广阔。本项目旨在运用分子印迹技术改善电化学发光中存在的选择性差的问题，通过溶胶-凝胶法、电聚合法等方法合成出毒性低、发光效率高、稳定性强的电化学发光分子印迹聚合物。构建新型电化学发光能量转移模式，建立灵敏度高、特异性好的分子印迹电化学发光能量转移分析方法，探索其在爆炸物和抗肿瘤药物等方面的分析应用。从而揭示电化学发光能量转移的传感机制，为电化学发光体系的探索和复杂样品中的小分子的检测奠定基础。

本项目成功合成了鲁米诺掺杂的二氧化硅纳米颗粒、吡啶钌掺杂的二氧化硅纳米颗粒、邻菲啰啉钌掺杂的二氧化硅纳米颗粒、发光的硅纳米颗粒、戊二醛-壳聚糖非共轭荧光聚合物（GCPF）和发光金纳米簇等6种发光材料，利用发光给体和受体的光谱重叠原理，建立6种能量转移模式，建立了简单、快速、灵敏、选择性好的分析检测方法，实现了能量转移在爆炸物TNP、葡萄糖、药物盐酸利多卡因和姜黄素、臭氧分析中的应用，推动了发光纳米颗粒和电化学发光能量转移技术在爆炸物检测、药物检测等领域的应用。